Archive
Current Protocols in Molecular Biology Supplements
2010: Supplement
89
2009: Supplement 88
Supplement 87
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2005: Supplement 72
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2000: Supplement 52
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1996: Supplement 36
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1992: Supplement 20
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1991: Supplement 16
Supplement 15
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1990: Supplement 12
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1989: Supplement 8
Supplement
7 Supplement 6
Supplement 5
1988: Supplement 4
Supplement
3 Supplement 2
Supplement 1
1987: Core publication
Supplement 89,
January 2010
Unit 4.11 RNA-Seq: A Method for Comprehensive Transcriptome
Analysis
Abstract | Full Text:
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PDF
Unit 17.13A Endoglycosidase and Glycoamidase Release from
N-Linked Glycans
Abstract | Full Text:
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PDF
Unit 19.10 Galaxy: A Web-Based Genome Analysis Tool for
Experimentalists
Abstract | Full Text:
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PDF
Unit 21.15 Chromatin Interaction Analysis Using Paired-End Tag
Sequence
Abstract | Full Text:
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PDF
Unit 21.16 Mapping Networks of Protein-Mediated Physical
Interactions Between Chromatin Elements
Abstract | Full Text:
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PDF
Unit 21.17 Methylation-Sensitive Single-Molecule Analysis of
Chromatin Structure
Abstract | Full Text:
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PDF
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Supplement 88,
October 2009
Unit 10.25 Preparation of Proteins and Peptides for Mass
Spectrometry Analysis in a Bottom-Up Proteomics Workflow
Abstract | Full Text:
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PDF
Unit 15.9 Heat-Activatable Primers for Hot-Start PCR and
Hot-Start One-Step RT-PCR: Endpoint and Real-Time
Experiments
Abstract | Full Text:
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PDF
Unit 19.9 The UCSC Genome Browser: What Every Molecular
Biologist Should Know
Abstract | Full Text:
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PDF
Unit 24.2 Design, Synthesis, and Amplification of DNA Pools for
In Vitro Selection
Abstract | Full Text:
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PDF
Unit 24.3 In Vitro Selection of RNA Aptamers to a Protein Target
by Filter Immobilization
Abstract | Full Text:
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PDF
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Supplement 87,
July 2009
Unit 17.4 Metabolic Radiolabeling of Animal Cell
Glycoconjugates
Abstract | Full Text:
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PDF
Unit 19.8 Resources for Small Regulatory RNAs
Abstract | Full Text:
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Unit 24.6 Directed Evolution of Proteins In Vitro Using
Compartmentalization in Emulsions
Abstract | Full Text:
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Unit 25A.3 Laser Microdissection-Mediated Isolation and In Vitro
Transcriptional Amplification of Plant RNA
Abstract | Full Text:
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PDF
Appendix 6 Pathway Modulators and Inhibitors
Abstract | Full Text:
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PDF
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Supplement 86,
April 2009
Unit 3.21 DNA Cloning and Engineering by Uracil
Excision
Abstract | Full Text:
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Unit 9.5 Selection of Transfected Mammalian Cells
Abstract | Full Text:
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Unit 19.7 Using PATIMDB to Create Bacterial Transposon Insertion
Mutant Libraries
Abstract | Full Text:
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PDF
Unit 20.7 Two-Hybrid Dual Bait System
Abstract | Full Text:
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PDF
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Supplement 85,
January 2009
Unit 10.6 Staining Proteins in Gels
Abstract | Full Text:
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PDF
Unit 22.4 Overview of mRNA Expression Profiling Using DNA
Microarrays
Abstract | Full Text:
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PDF
Unit 22.5 Pattern Discovery in Expression Profiling
Data
Abstract | Full Text:
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PDF
Unit 23.13 Recombineering-Based Procedure for Creating
Cre/loxP Conditional Knockouts in the Mouse
Abstract | Full Text:
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PDF
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Supplement 84,
October 2008
Unit 3.5 DNA-Dependent DNA Polymerases
Abstract | Full Text:
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Unit 3.6 Template-Independent DNA Polymerases
Abstract | Full Text:
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Unit 3.7 RNA-Dependent DNA Polymerases
Abstract | Full Text:
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Unit 3.8 RNA Polymerases
Abstract | Full Text:
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Unit 3.9 DNA Repair Enzymes
Abstract | Full Text:
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Unit 3.13 Ribonucleases
Abstract | Full Text:
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Unit 3.15 RNA Ligases
Abstract | Full Text:
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Unit 10.7 Detection of Proteins on Blot Transfer
Membranes
Abstract | Full Text:
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PDF
Unit 14.18 Using Cell-ID 1.4 with R for Microscope-Based
Cytometry
Abstract | Full Text:
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PDF
Unit 14.19 Visualization of Microscopy-Based Spectral Imaging
Data from Multi-Label Tissue Sections
Abstract | Full Text:
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Unit 29B.5 Dietary Manipulation of Mouse Metabolism
Abstract | Full Text:
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Supplement 83,
July 2008
Unit 10.8 Immunoblotting and Immunodetection
Abstract | Full Text:
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PDF
Unit 11.10 Production of Monoclonal Antibody Supernatant and
Ascites Fluid
Abstract | Full Text:
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PDF
Unit 26.8 Using Morpholinos to Control Gene Expression
Abstract | Full Text:
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PDF
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Supplement 82,
April 2008
Unit 13.2 Growth and Manipulation of Yeast
Abstract | Full Text:
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PDF
Unit 13.3B EMS and UV Mutagenesis in Yeast
Abstract | Full Text:
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PDF
Unit 14.8 In Situ Polymerase Chain Reaction and Hybridization to
Detect Low-Abundance Nucleic Acid Targets
Abstract | Full Text:
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PDF
Unit 14.17 Using CellProfiler for Automatic Identification and
Measurement of Biological Objects in Images
Abstract | Full Text:
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PDF
Unit 20.1 Interaction Trap/Two-Hybrid System to Identify
Interacting Proteins
Abstract | Full Text:
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PDF
Unit 23.5 Production of a Heterozygous Mutant Cell Line by
Homologous Recombination (Single Knockout)
Abstract | Full Text:
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PDF
Unit 23.6 Production of a Homozygous Mutant Embryonic Stem Cell
Line (Double Knockout)
Abstract | Full Text:
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PDF
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Supplement 81,
January 2008
Unit 7.1 Overview of DNA Sequencing Strategies
Abstract | Full Text:
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PDF
Unit 9.4 Tranfection of Cultured Eukaryotic Cells Using Cationic
Lipid Reagents
Abstract | Full Text:
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PDF
Unit 11.12 Production of Polyclonal Antisera
Abstract | Full Text:
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PDF
Unit 14.6 Immunohistochemistry
Abstract | Full Text:
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Unit 14.11 Basic Confocal Microscopy
Abstract | Full Text:
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Unit 26.7 Analysis of Small Endogenous RNAs
Abstract | Full Text:
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Supplement 80,
October 2007
Unit 21.14 Chromosome Conformation Capture Carbon Copy
Technology
Abstract | Full Text:
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PDF
Unit 24.1 Overview of Receptors from Combinatorial Nucleic Acid
and Protein Libraries
Abstract | Full Text:
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PDF
Unit 25B.6 Serial Analysis of Gene Expression (SAGE):
Experimental Method and Data Analysis
Abstract | Full Text:
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PDF
Unit 29A.4 Tissue Collection for Systematic Phenotyping in the
Mouse
Abstract | Full Text:
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PDF
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Supplement 79,
July 2007
Unit 1.17 E. coli Genome Manipulation by P1
Transduction
Abstract | Full Text:
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PDF
Unit 14.7 In Situ Hybridization and Detection Using Nonisotopic
Probes
Abstract | Full Text:
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PDF
Unit 21.12 Paired-End diTagging for Transcriptome and Genome
Analysis
Abstract | Full Text:
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PDF
Unit 21.13 ChIP-chip for Genome-Wide Analysis of Protein Binding
in Mammalian Cells
Abstract | Full Text:
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PDF
Appendix 1E Radioactivity
Abstract | Full Text:
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Appendix 1F Safe Use of Radioisotopes
Abstract | Full Text:
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[
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Supplement 78,
April 2007
Unit 1.16 Recombineering: Genetic Engineering in Bacteria
Using Homologous Recombination
Abstract | Full Text:
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PDF
Unit 3.17 Constructing Recombinant DNA Molecules by
PCR
Abstract | Full Text:
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PDF
Unit 4.10 Identification of Newly Transcribed RNA
Abstract | Full Text:
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PDF
Unit 16.25 Production of Recombinant Adeno-Associated Viral
Vectors for In Vitro and In Vivo Use
Abstract | Full Text:
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PDF
Unit 29B.4 Histopathology in Mouse Metabolic
Investigations
Abstract | Full Text:
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PDF
[
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Supplement 77,
January 2007
Unit 18.13 Isolation of Phosphopeptides by Immobilized Metal
Ion Affinity Chromatography
Abstract | Full Text:
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PDF
Unit 19.6 Analysis and Management of Microarray Gene Expression
Data
Abstract | Full Text:
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Unit 29B.3 Evaluation of Glucose Homeostasis
Abstract | Full Text:
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Supplement 76,
October 2006
Unit 7.8 Polony DNA Sequencing
Abstract | Full Text:
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Unit 10.0 Introduction to Analysis of Proteins
Abstract | Full Text:
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Unit 10.1A Spectrophotometric and Colorimetric Determination of
Protein Concentration
Abstract | Full Text:
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Unit 20.5 Detection of Protein-Protein Interactions by
Coprecipitation
Abstract | Full Text:
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PDF
Unit 23.1 Overview of Gene Targeting by Homologous
Recombination
Abstract | Full Text:
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PDF
Appendix 3D Quantitation of DNA and RNA with Absorption and
Fluorescence Spectroscopy
Abstract | Full Text:
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PDF
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Supplement 75,
July 2006
Unit 10.2A One-Dimensional SDS Gel Electrophoresis of
Proteins
Abstract | Full Text:
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PDF
Unit 12.1 Preparation of Nuclear and Cytoplasmic Extracts from
Mammalian Cells
Abstract | Full Text:
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PDF
Unit 21.12 Paired-End diTagging for Transcriptome and Genome
Analysis
Full Text:
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PDF
Unit 29A.3 Collection of Blood and Plasma from the
Mouse
Abstract | Full Text:
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Unit 29B.2 Lipid and Bile Acid Analysis
Abstract | Full Text:
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Supplement 74,
April 2006
Unit 3.20 Recombinational Cloning
Abstract | Full Text:
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Unit 10.24 Solution Radioimmunoassay of Proteins and
Peptides
Abstract | Full Text:
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Unit 14.16 Three-Dimensional Reconstruction of Tissues
Abstract | Full Text:
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PDF
Unit 21.11 Mapping Chromatin Interactions by Chromosome
Conformation Capture
Abstract | Full Text:
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PDF
Appendix 3F Techniques for Mammalian Cell Tissue
Culture
Abstract | Full Text:
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[
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Supplement 73,
January 2006
Unit 15.8 High-Throughput Real-Time Quantitative Reverse
Transcription PCR
Abstract | Full Text:
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PDF
Unit 18.12 The Detection of MAPK Signaling
Abstract | Full Text:
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Unit 27.4 RNA Immunoprecipitation for Determining RNA-Protein
Associations In Vivo
Abstract | Full Text:
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PDF
Unit 29A.1 Uses of Forward and Reverse Genetics in Mice to Study
Gene Function
Abstract | Full Text:
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PDF
Unit 29A.2 Minimizing Variation Due to Genotype and
Environment
Abstract | Full Text:
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Unit 29B.1 Evaluation of Energy Homeostasis
Abstract | Full Text:
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[
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Supplement 72,
October 2005
Unit 10.24 Solution Radioimmunoassay of Proteins and
Peptides
Full Text:
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PDF
Unit 21.10 Identifying Chromosomal Targets of DNA-Binding
Proteins by Sequence Tag Analysis of Genomic Enrichment
(STAGE)
Abstract | Full Text:
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Unit 26.4 Cloning of Small RNA Molecules
Abstract | Full Text:
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Unit 26.6 RNAi in Transgenic Plants
Abstract | Full Text:
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[
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Supplement 71,
July 2005
Unit 15.8 High-Throughput Real-Time Quantitative Reverse
Transcription PCR
Full Text:
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PDF
Unit 23.10 Transgenic Mouse Colony Management
Abstract | Full Text:
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Unit 23.11 Modification and Production of BAC
Transgenes
Abstract | Full Text:
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Unit 23.12 Regulation of Transgene Expression Using
Tetracycline
Abstract | Full Text:
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Unit 28.3 Establishment and Culture of Human Skin
Fibroblasts
Abstract | Full Text:
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[
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Supplement 70,
April 2005
Unit 1.16 Recombineering: Genetic Engineering in Bacteria
Using Homologous Recombination
Full Text:
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PDF
Unit 19.5 Basic Protein Sequence Analysis
Abstract | Full Text:
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Unit 21.8 Analysis of Protein Co-Occupancy by Quantitative
Sequential Chromatin Immunoprecipitation
Abstract | Full Text:
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PDF
Unit 28.1 Preparation, Culture, and Immortalization of Mouse
Embryonic Fibroblasts
Abstract | Full Text:
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PDF
Unit 28.2 Isolation and Immortalization of Lymphocytes
Abstract | Full Text:
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Appendix 1A Common Abbreviations
Abstract |Full Text:
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[
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Supplement 69,
January 2005
Unit 10.23 Difference Gel Electrophoresis (DIGE) Using CyDye
DIGE Fluor Minimal Dyes
Abstract | Full Text:
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PDF
Unit 16.24 Helper-Dependent Adenoviral Vectors
Abstract | Full Text:
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PDF
Unit 21.1 Micrococcal Nuclease Analysis of Chromatin
Structure
Abstract | Full Text:
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PDF
Unit 21.3 Chromatin Immunoprecipitation for Determining the
Association of Proteins with Specific Genomic Sequences In
Vivo
Abstract | Full Text:
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PDF
Unit 22.5 Pattern Discovery in Expression Profiling
Data
Full Text:
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PDF
[
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Supplement 68,
October 2004
Unit 2.8 Capillary Electrophoresis of DNA
Abstract | Full Text:
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Unit 19.4 Protein Databases on the Internet
Abstract | Full Text:
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PDF
Unit 21.8 Analysis of Protein Co-Occupancy by Quantitative
Sequential Chromatin Immunoprecipitation
Full Text:
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PDF
Unit 21.9 Defining In Vivo Targets of Nuclear Proteins by
Chromatin Immunoprecipitation and Microarray Analysis
Abstract | Full Text:
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PDF
Unit 23.9 Transgenic Mouse Production By Zygote
Injection
Abstract | Full Text:
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[
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Supplement 67,
July 2004
Unit 4.9 Analysis of RNA by Northern and Slot Blot
Hybridization
Abstract | Full Text:
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PDF
Unit 10.4 Two-Dimensional Gel Electrophoresis
Abstract | Full Text:
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PDF
Unit 18.12 The Detection of MAPK Signaling
Full Text:
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PDF
Unit 22.4 Overview of mRNA Expression Profiling Using
Microarrays
Full Text:
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PDF
[
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Supplement 66,
April 2004
Unit 10.5 Overview of Digital Electrophoresis
Analysis
Abstract | Full Text:
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PDF
Unit 10.8 Immunoblotting and Immunodetection
Full Text:
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PDF
Unit 14.9 Whole-Mount In Situ Hybridization and Detection of RNA
s in Vertebrate Embryos and Isolated Organs
Abstract | Full Text:
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PDF
Unit 18.11 Design and Use of Analog-Sensitive Protein
Kinases
Abstract | Full Text:
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PDF
Appendix 3D Quantitation of DNA and RNA with Absorption and
Fluorescence Spectroscopy
Full Text:
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PDF
[
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Supplement 65,
January 2004
Unit 16.9 Overview of the Baculovirus Expression
System
Abstract | Full Text:
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PDF
Unit 16.10 Maintenance of Insect Cell Cultures and Generation of
Recombinant Baculoviruses
Abstract | Full Text:
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PDF
Unit 16.11 Expression and Purification of Recombinant Proteins
Using the Baculovirus System
Abstract | Full Text:
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PDF
Unit 20.8 Interaction Trap/Two-Hybrid System to Identify
Loss-of-Interaction Mutant Proteins
Abstract | Full Text:
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PDF
Unit 21.3 Chromatin Immunoprecipitation for Determining the
Association of Proteins with Specific Genomic Sequences In
Vivo
Full Text:
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PDF
Unit 26.5 RNA Interference in Cultured Drosophila
Cells
Abstract | Full Text:
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PDF
[
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Supplement 64,
October 2003
Unit 10.7 Detection of Proteins on Blot Transfer
Membranes
Full Text:
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PDF
Unit 13.14 Overview of Schizosaccharomyces pombe
Abstract | Full Text:
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Unit 13.15 S. pombe Strain
Maintenance and Media
Abstract | Full Text:
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Unit 13.16 Growth and Manipulation of S.
pombe
Abstract | Full Text:
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Unit 13.17 Introduction of DNA into S.
pombe Cells
Abstract | Full Text:
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Unit 26.4 Cloning of Small RNA Molecules
Full Text:
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Supplement 63,
July 2003
Unit 10.6 Staining Proteins in Gels
Full Text:
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PDF
Unit 14.15 Overview of Image Analysis, Image Importing, and
Image Processing Using Freeware
Abstract | Full Text:
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PDF
Unit 27.1 Agarose Gel Separation/Isolation of RNA-Protein
Complexes
Abstract | Full Text:
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PDF
Unit 27.2 Identification of RNA Binding Proteins by UV
Cross-Linking
Abstract | Full Text:
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Unit 27.3 Purification of Functional RNA-Protein Complexes Using
MS 2-MBP
Abstract | Full Text:
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PDF
[
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Supplement 62,
April 2003
Unit 9.15 Human Somatic Cell Gene Targeting
Abstract | Full Text:
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PDF
Unit 18.10 Use of Protein Phosphatase Inhibitors
Abstract | Full Text:
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PDF
Unit 26.1 Overview of RNA Interference and Related
Processes
Abstract | Full Text:
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PDF
Unit 26.2 Gene Silencing by RNAi in Mammalian Cells
Abstract | Full Text:
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PDF
Unit 26.3 RNA Interference in Caenorhabditis Elegans
Abstract | Full Text:
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PDF
Appendix 1A Common Abbreviations
Full Text:
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PDF
[
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Supplement 61,
January 2003
Unit 1.16 Recombineering: Genetic Engineering in Bacteria
Using Homologous Recombination
Full Text:
HTML
PDF
Unit 21.3 Chromatin Immunoprecipitation for Determining the
Association of Proteins with Specific Genomic Sequences In
Vivo
Unit 25B.6 Serial Analysis of Gene Expression (SAGE)
Full Text:
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PDF
Unit 25B.8 Gene Expression Analysis of a Single or Few
Cells
Abstract | Full Text:
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PDF
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Supplement 60,
October 2002
Unit 16.12 Transient Expression of Proteins Using COS
Cells
Abstract | Full Text:
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PDF
Unit 16.13 Expression and Purification of Epitope-Tagged
Multisubunit Protein Complexes from Mammalian Cells
Abstract | Full Text:
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PDF
Unit 16.14 Inducible Gene Expression Using an Autoregulatory,
Tetracycline-Controlled System
Abstract | Full Text:
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PDF
Unit 16.21 Overview of the HIV-1 Lentiviral Vector
System
Abstract | Full Text:
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PDF
Unit 16.22 Generation of HIV-1-Based Lentiviral Vector
Particles
Abstract | Full Text:
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PDF
Unit 16.23 Amplification Using CHO Cell Expression
Vectors
Abstract | Full Text:
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PDF
Unit 20.7 Two-Hybrid Dual Bait System
Full Text:
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PDF
Unit 25B.7 Representational Difference Analysis
Abstract | Full Text:
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Supplement 59,
July 2002
Unit 1.1 Media Preparation and Bacteriological
Tools
Abstract | Full Text:
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PDF
Unit 1.2 Growth in Liquid Media
Abstract | Full Text:
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Unit 1.3 Growth on Solid Media
Abstract | Full Text:
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Unit 1.4 Selected Topics from Classical Bacterial
Genetics
Abstract | Full Text:
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Unit 2.1A Purification and Concentration of DNA from Aqueous
Solutions
Abstract | Full Text:
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PDF
Unit 2.6 Isolation and Purification of Large DNA Restriction
Fragments from Agarose Gels
Abstract | Full Text:
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PDF
Unit 11.15 Introduction to Peptide Synthesis
Abstract | Full Text:
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Unit 11.16 Synthetic Peptides for Production of Antibodies that
Recognize Intact Proteins
Abstract | Full Text:
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Appendix 1K Compendium of Drugs Commonly Used in Molecular
Biology Research
Abstract | Full Text:
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PDF
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Supplement 58,
April 2002
Unit 4.1 Preparation of Cytoplasmic RNA from Tissue Culture
Cells
Abstract | Full Text:
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PDF
Unit 21.7 Chromatin Assembly Using Drosophila Systems
Abstract | Full Text:
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PDF
Unit 25A.2 Preparation of Single Cells from Solid Tissues for
Analysis by PCR
Abstract | Full Text:
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PDF
Appendix 1H Safe Use of Hazardous Chemicals
Abstract | Full Text:
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PDF
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Supplement 57,
January 2002
Unit 17.6 Detection and Analysis of Proteins Modified by
O-Linked N-Acetylglucosamine
Abstract | Full Text:
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PDF
Unit 23.8 Mouse Colony Management
Abstract | Full Text:
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PDF
Unit 25B.5 AFLP-Based Transcript Profiling
Abstract | Full Text:
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PDF
Unit 25B.6 Serial Analysis of Gene Expression
(SAGE)
[
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Supplement 56,
October 2001
Unit 15.1 Enzymatic Amplification of DNA by PCR: Standard
Procedures and Optimization
Abstract | Full Text:
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PDF
Unit 15.2 Direct DNA Sequencing of PCR Products
Abstract | Full Text:
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PDF
Unit 15.3 Ligation-Mediated PCR for Genomic Sequencing and
Footprinting
Abstract | Full Text:
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PDF
Unit 15.4 Molecular Cloning of PCR Products
Abstract | Full Text:
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Unit 15.5 Enzymatic Amplification of RNA by PCR
(RT-PCR)
Abstract | Full Text:
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Unit 15.7 Quantitation of Rare DNAs by PCR
Abstract | Full Text:
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Unit 25B.3 Differential Display of mRNA by PCR
Abstract | Full Text:
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Unit 25B.4 Restriction-Mediated Differential Display
(RMDD)
Abstract | Full Text:
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PDF
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Supplement 55,
July 2001
Unit 5.9 Construction of Bacterial Artificial Chromosome
(BAC/PAC) Libraries
Abstract | Full Text:
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PDF
Unit 12.12 Yeast One-Hybrid Screening for DNA-Protein
Interactions
Abstract | Full Text:
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PDF
Unit 14.3 In Situ Hybridization to Cellular RNA
Abstract | Full Text:
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PDF
Unit 20.6 Identification of Protein Interactions by Far Western
Analysis
Abstract | Full Text:
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PDF
Unit 25A.1 Laser Capture Microdissection
Abstract | Full Text:
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PDF
Unit 25B.1 Production of a Subtracted cDNA Library
Abstract | Full Text:
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PDF
Unit 25B.2 PCR-Based Subtractive cDNA Cloning
Abstract | Full Text:
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PDF
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Supplement 54,
April 2001
Unit 10.12 HPLC of Peptides and Proteins: Preparation and
System Set-Up
Abstract | Full Text:
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PDF
Unit 10.13 HPLC of Peptides and Proteins: Standard Operating
Conditions
Abstract | Full Text:
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PDF
Unit 10.14 Reversed-Phase Isolation of Peptides
Abstract | Full Text:
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PDF
Unit 16.22 Generation of HIV-1-Based Lentiviral Vector
Particles
Unit 21.6 Assembly of Nucleosomal Templates by Salt
Dialysis
Abstract | Full Text:
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PDF
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Supplement 53,
January 2001
Unit 11.18 Identification of Polyol-Responsive Monoclonal
Antibodies for Use in Immunoaffinity Chromatography
Abstract | Full Text:
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PDF
Unit 22.3 Profiling Human Gene Expression with cDNA
Microarrays
Abstract | Full Text:
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PDF
Unit 23.7 Chimeric Mouse Production by Microinjection
Abstract | Full Text:
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PDF
Unit 24.5 Protein Selection Using mRNA Display
Abstract | Full Text:
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PDF
[
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Supplement 52,
October 2000
Unit 23.4 Mouse Embryonic Stem (ES) Cell Isolation
Abstract | Full Text:
HTML
PDF
Unit 23.5 Production of a Heterozygous Mutant Cell Line by
Homologous Recombination (Single Knockout)
Full Text:
HTML
PDF
Unit 23.6 Production of a Homozygous Mutant Embryonic Stem Cell
Line (Double Knockout)
Full Text:
HTML
PDF
Unit 24.2 Design, Synthesis, and Amplification of DNA Pools for
Construction of Combinatorial Pools and Libraries
Full Text:
HTML
PDF
Unit 24.3 In Vitro Selection of RNA Aptamers to a Protein Target
by Filter Immobilization
Full Text:
HTML
PDF
Unit 24.4 Peptide Aptamers: Dominant "Genetic" Agents for
Forward and Reverse Analysis of Cellular Processes
Abstract | Full Text:
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PDF
[
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Supplement 51,
July 2000
Unit 2.5A Agarose Gel Electrophoresis
Abstract | Full Text:
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PDF
Unit 2.5B Pulsed-Field Gel Electrophoresis
Abstract | Full Text:
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PDF
Unit 8.3 Random Mutagenesis by PCR
Abstract | Full Text:
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PDF
Unit 10.6 Staining Proteins in Gels
Unit 13.3 Genome-Wide Transposon Mutagenesis in Yeast
Abstract | Full Text:
HTML
PDF
Unit 19.2 Sequence Databases: Integrated Information Retrieval
and Data Submission
Abstract | Full Text:
HTML
PDF
Unit 23.1 Overview of Gene Targeting by Homologous
Recombination
Full Text:
HTML
PDF
Unit 23.2 Mouse Embryo Fibroblast (MEF) Feeder Cell
Preparation
Abstract | Full Text:
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PDF
Unit 23.3 Mouse Embryonic Stem (ES) Cell Culture
Abstract | Full Text:
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PDF
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Supplement 50,
April 2000
Unit 11.13 In Vitro Antibody Production
Abstract | Full Text:
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PDF
Unit 11.14 Purification of Immunoglobulin G Fraction from
Antiserum, Ascites Fluid, or Hybridoma Supernatant
Abstract | Full Text:
HTML
PDF
Unit 11.17 Determination of the Specific Antibody
Titer
Abstract | Full Text:
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PDF
Unit 14.14 Whole-Mount Histochemical Detection of
β-Galactosidase Activity
Abstract | Full Text:
HTML
PDF
Unit 19.1 Internet Basics for Biologists
Abstract | Full Text:
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PDF
Unit 19.4 Protein Databases on the Internet
Full Text:
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PDF
Unit 21.5 Isolation of Histones and Nucleosome Cores from
Mammalian Cells
Abstract | Full Text:
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Supplement 49,
January 2000
Unit 14.12 Measurement of In Situ Hybridization
Abstract | Full Text:
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PDF
Unit 14.13 Morphological, Biochemical, and Flow Cytometric
Assays of Apoptosis
Abstract | Full Text:
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PDF
Unit 22.1 Overview of Nucleic Acid Arrays
Abstract | Full Text:
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PDF
Unit 22.2 Preparation of mRNA for Expression
Monitoring
Abstract | Full Text:
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PDF
Appendix 1J Common Conversion Factors
Abstract | Full Text:
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Supplement 48,
October 1999
Unit 9.4 Transfection of Cultured Eukaryotic Cells Using
Cationic Lipid Reagents
Full Text:
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PDF
Unit 10.16 Immunoprecipitation
Abstract | Full Text:
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PDF
Unit 17.13A Endoglycosidase and Glycoamidase Release of N-Linked
Oligosaccharides
Full Text:
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PDF
Unit 18.9 Phosphopeptide Mapping and Identification of
Phosphorylation Sites
Abstract | Full Text:
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PDF
Unit 21.4 DN ase I and Hydroxyl Radical Characterization of
Chromatin Complexes
Abstract | Full Text:
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PDF
Appendix 3A Detection and Quantitation of Radiolabeled Proteins
and DNA in Gels and Blots
Abstract | Full Text:
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PDF
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Supplement 47,
July 1999
Unit 7.0 Introduction to DNA Sequencing
Abstract | Full Text:
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PDF
Unit 7.4A DNA Sequencing by the Dideoxy Method
Abstract | Full Text:
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PDF
Unit 7.4B Dideoxy DNA Sequencing with Chemiluminescent
Detection
Abstract | Full Text:
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PDF
Unit 10.2A One-Dimensional SDS Gel Electrophoresis of
Proteins
Full Text:
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PDF
Unit 10.2B One-Dimensional Electrophoresis Using Nondenaturing
Conditions
Abstract | Full Text:
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Unit 21.3 Chromatin Immunoprecipitation for Determining the
Association of Proteins with Specific Genomic Sequences In
Vivo
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Supplement 46,
April 1999
Unit 7.1 DNA Sequencing Strategies
Full Text:
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PDF
Unit 15.1 Enzymatic Amplification of DNA by PCR: Standard
Procedures and Optimization
Unit 19.3 Sequence Similarity Searching Using the BLAST Family
of Programs
Abstract | Full Text:
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PDF
Unit 20.1 Interaction Trap/Two-Hybrid System to Identify
Interacting Proteins
Full Text:
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PDF
Unit 20.5 Detection of Protein-Protein Interactions by
Coprecipitation
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PDF
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Supplement 45,
January 1999
Unit 2.7 Separation of Small DNA Fragments by Conventional
Gel Electrophoresis
Abstract | Full Text:
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PDF
Unit 2.8 Capillary Electrophoresis of DNA
Full Text:
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PDF
Unit 9.15 Human Somatic Cell Gene Targeting
Abstract | Full Text:
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Unit 9.16 Production of Heterozygous Mutant Cell Line
Unit 9.17 Production of Homozygous Mutant
Unit 21.1 Micrococcal Nuclease Analysis of Chromatin
Structure
Full Text:
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PDF
Unit 21.2 Separation of Histone Variants and
Post-Translationally Modified Isoforms by Triton/Acetic Acid/Urea
Polyacrylamide Gel Electrophoresis
Abstract | Full Text:
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Supplement 44,
October 1998
Unit 10.9 Gel-Filtration Chromatography
Abstract | Full Text:
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Unit 10.10 Ion-Exchange Chromatography
Abstract | Full Text:
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Unit 10.18 Metabolic Labeling with Amino Acids
Abstract | Full Text:
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Unit 14.10 Principles and Application of Fluorescence
Microscopy
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Unit 14.11 Basic Confocal Microscopy
Full Text:
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Supplement 43,
July 1998
Unit 10.12 HPLC of Peptides and Proteins: Preparation and
System Set-Up
Unit 16.13 Expression and Purification of Epitope-Tagged
Multisubunit Protein Complexes from Mammalian Cells
Unit 16.15 Overview of the Vaccinia Virus Expression
System
Abstract | Full Text:
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PDF
Unit 16.16 Preparation of Cell Cultures and Vaccinia Virus
Stocks
Abstract | Full Text:
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PDF
Unit 16.17 Generation of Recombinant Vaccinia Viruses
Abstract | Full Text:
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PDF
Unit 16.18 Characterization of Recombinant Vaccinia Viruses and
Their Products
Abstract | Full Text:
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PDF
Unit 16.19 Gene Expression Using the Vaccinia Virus/T7 RNA
Polymerase Hybrid System
Abstract | Full Text:
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Appendix 1I Commonly Used Detergents
Abstract | Full Text:
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Appendix 3I Statistics for the Molecular Biologist: Group
Comparisons
Abstract | Full Text:
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Supplement 42,
April 1998
Unit 2.1A Purification and Concentration of DNA from Aqueous
Solutions
Unit 2.1B Purification of DNA by Anion-Exchange
Chromatography
Abstract | Full Text:
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PDF
Unit 2.11 Synthesis and Purification of
Oligonucleotides
Abstract | Full Text:
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PDF
Unit 2.12 Purification of Oligonucleotides Using Denaturing
Polyacrylamide Gel Electrophoresis
Abstract | Full Text:
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PDF
Unit 19.1 Internet Basics for Biologists
Unit 19.2 Sequence Databases: Integrated Information Retrieval
and Data Submission
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Page]
Supplement 41,
January 1998
Unit 1.5 Introduction to Plasmid Biology
Abstract | Full Text:
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PDF
Unit 1.7 Large-Scale Preparation of Plasmid DNA
Abstract | Full Text:
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PDF
Unit 10.5 Overview of Digital Electrophoresis Analysis
Unit 10.15 Purification of Recombinant Proteins and Study of
Protein Interaction by Epitope Tagging
Abstract | Full Text:
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PDF
Unit 10.22 Protein Identification and Characterization by Mass
Spectrometry
Abstract | Full Text:
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Supplement 40,
October 1997
Unit 9.2 Transfection Using DEAE-Dextran
Abstract | Full Text:
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PDF
Unit 18.7 Assays of Protein Kinases Using Exogenous
Substrates
Abstract | Full Text:
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PDF
Unit 18.8 Permeabilization Strategies to Study Protein
Phosphorylation
Abstract | Full Text:
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PDF
Unit 20.4 Surface Plasmon Resonance for Measurements of
Biological Interest
Abstract | Full Text:
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PDF
Appendix 3G Importing Biological Materials
Abstract | Full Text:
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PDF
Appendix 3H Kinetic Assay Methods
Abstract | Full Text:
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Supplement 39,
July 1997
Unit 9.5 Selection of Transfected Mammalian Cells
Full Text:
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PDF
Unit 10.8 Immunoblotting and Immunodetection
Unit 13.6 Yeast Vectors and Assays for Expression of Cloned
Genes
Abstract | Full Text:
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PDF
Unit 13.9 Manipulation of Plasmids from Yeast Cells
Abstract | Full Text:
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PDF
Unit 13.10 Manipulation of Cloned Yeast DNA
Abstract | Full Text:
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Unit 20.1 Interaction Trap/Two-Hybrid System to Identify
Interacting Proteins
Unit 20.3 Phage-Based Expression Cloning to Identify Interacting
Proteins
Abstract | Full Text:
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PDF
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Supplement 38,
April 1997
Unit 10.21 Overview of Peptide and Protein Analysis by Mass
Spectrometry
Abstract | Full Text:
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PDF
Unit 16.9 Overview of the Baculovirus Expression
System
Unit 16.10 Maintenance of Insect Cell Cultures and Generation of
Recombinant Baculoviruses
Unit 16.11 Expression and Purification of Recombinant Proteins
Using the Baculovirus System
Unit 16.21 Overview of the HIV-1 Lentiviral Vector
System
Appendix 1C Characteristics of Amino
Acids
Abstract|FullText:
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Supplement 37,
January 1997
Unit 1.8 Introduction of Plasmid DNA into Cells
Abstract | Full Text:
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PDF
Unit 4.9 Analysis of RNA by Northern and Slot Blot
Hybridization
Full Text:
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PDF
Unit 8.5 Directed Mutagenesis Using the Polymerase Chain
Reaction
Abstract | Full Text:
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PDF
Unit 9.12 Large-Scale Preparation and Concentration of
Retrovirus Stocks
Abstract | Full Text:
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PDF
Unit 11.12 In Vitro Antibody Production
Full Text:
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Supplement 36,
October 1996
Unit 4.2 Guanidine Methods for Total RNA
Preparation
Abstract | Full Text:
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PDF
Unit 9.1 Calcium Phosphate Transfection
Abstract | Full Text:
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PDF
Unit 9.9 Overview of the Retrovirus Transduction
System
Abstract | Full Text:
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PDF
Unit 9.10 Preparation of a Specific Retrovirus Producer Cell
Line
Abstract | Full Text:
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PDF
Unit 9.11 Transient Transfection Methods for Preparation of
High-Titer Retroviral Supernatants
Abstract | Full Text:
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PDF
Unit 9.13 Detection of Helper Virus in Retrovirus
Stocks
Abstract | Full Text:
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PDF
Unit 9.14 Retrovirus Infection of Cells In Vitro and In
Vivo
Abstract | Full Text:
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PDF
Unit 10.11A Immunoaffinity Chromatography
Abstract | Full Text:
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PDF
Unit 10.11B Metal-Chelate Affinity Chromatography
Abstract | Full Text:
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PDF
Unit 12.2 Mobility Shift DNA-Binding Assay Using Gel
Electrophoresis
Abstract | Full Text:
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PDF
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Supplement 35,
July 1996
Unit 10.1A Spectrophotometric and Colorimetric Determination
of Protein Concentration
Full Text:
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PDF
Unit 10.1B Quantitative Amino Acid Analysis
Abstract | Full Text:
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PDF
Unit 14.9 Whole-Mount In Situ Hybridization and Detection of RNA
s in Vertebrate Embryos and Isolated Organs
Unit 18.6 Production of Antibodies That Recognize Specific
Tyrosine-Phosphorylated Peptides
Abstract | Full Text:
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PDF
Appendix 2 Commonly Used Reagents and Equipment
Abstract | Full Text:
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PDF
Appendix 3F Techniques for Mammalian Cell Tissue
Culture
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Supplement 34,
April 1996
Unit 5.9 Construction of Bacterial Artificial Chromosome
(BAC/PAC) Libraries
Unit 5.10 Amplification of a Bacteriophage Library
Abstract | Full Text:
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PDF
Unit 5.11 Amplification of Cosmid and Plasmid
Libraries
Abstract | Full Text:
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PDF
Unit 9.7C Use of the A. Victoria
Green Fluorescent Protein to Study Protein Dynamics in
Vivo
Abstract | Full Text:
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PDF
Unit 10.20 Capillary Electrophoresis of Proteins and
Peptides
Abstract | Full Text:
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PDF
Appendix 1H Safe Use of Hazardous Chemicals
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Supplement 33,
January 1996
Unit 12.11 Determination of Protein-DNA Sequence Specificity
by PCR-Assisted Binding-Site Selection
Abstract | Full Text:
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PDF
Unit 18.1 Overview of Protein Phosphorylation
Abstract | Full Text:
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PDF
Unit 18.5 Detection of Phosphorylation by Enzymatic
Techniques
Abstract | Full Text:
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PDF
Unit 20.1 Interaction Trap/Two-Hybrid System to Identify
Interacting Proteins
Unit 20.2 Affinity Purification of Proteins Binding to GST
Fusion Proteins
Abstract | Full Text:
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PDF
Appendix 1A Common Abbreviations
Appendix 1B Useful Measurements and Data
Abstract|FullText:
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Appendix 1C Characteristics of Amino Acids
Appendix 1D Characteristics of Nucleic Acids
Abstract|FullText:
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Appendix 1E Radioactivity
Full Text:
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Appendix 1F Safe Use of Radioisotopes
Full Text:
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Appendix 1G Centrifuges and Rotors
Abstract|FullText:
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of Page]
Supplement 32,
October 1995
Unit 15.7 Quantitation of Rare DNAs by PCR
Unit 17.9 Direct Chemical Analysis of Glycoconjugates for
Carbohydrates
Abstract | Full Text:
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PDF
Unit 17.11 Synthetic Glycosides as Primers of Oligosaccharide
Biosynthesis and Inhibitors of Glycoprotein and Proteoglycan
Assembly
Abstract | Full Text:
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PDF
Unit 17.10B Inhibition of Glycolipid Biosynthesis
Abstract | Full Text:
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Unit 17.14A Preparation of Glycopeptides
Abstract | Full Text:
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Unit 17.14B Detection of Individual Glycosylation Sites on
Glycoproteins
Abstract | Full Text:
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Unit 17.16 Acid Hydrolysis for Release of
Monosaccharides
Abstract | Full Text:
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PDF
Unit 17.17A Enzymatic Release of Oligosaccharides from
Glycolipids
Abstract | Full Text:
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PDF
Unit 17.17B Endo-β-Galactosidases and Keratanase
Abstract | Full Text:
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PDF
Unit 17.18 Analysis of Monosaccharides
Abstract | Full Text:
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PDF
Unit 17.19A Total Compositional Analysis by High-Performance
Liquid Chromatography or Gas-Liquid Chromatography
Abstract | Full Text:
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PDF
Unit 17.19B Composition of Labeled Monosaccharides from
Glycosaminoglycans
Abstract | Full Text:
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PDF
Unit 17.20 Analysis of Oligosaccharide Negative Charge by
Anion-Exchange Chromatography
Abstract | Full Text:
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PDF
Unit 17.21A HPLC Methods for the Fractionation and Analysis of
Negatively Charged Oligosaccharides and Gangliosides
Abstract | Full Text:
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PDF
Unit 17.21B Fractionation and Analysis of Neutral
Oligosaccharides by HPLC
Abstract | Full Text:
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Unit 17.22A Nitrous Acid Degradation of
Glycosaminoglycans
Abstract | Full Text:
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PDF
Unit 17.22B Analysis of Disaccharides and Tetrasaccharides
Released from Glycosaminoglycans
Abstract | Full Text:
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PDF
Unit 17.23 Analysis of Sulfate Esters by Solvolysis or
Hydrolysis
Abstract | Full Text:
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PDF
Unit 19.3 Sequence Similarity Searching Using the BLAST Family
of Programs
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Supplement 31,
July 1995
Unit 10.2 One-Dimensional SDS Gel Electrophoresis of
Proteins
Unit 14.7 In situ Hybridization and Detection Using Nonisotopic
Probes
Full Text:
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PDF
Unit 14.8 In situ Polymerase Chain Reaction and Hybridization to
Detect Low-Abundance Nucleic Acid Targets
Full Text:
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PDF
Unit 17.15B β-Elimination for Release of O -GalNAc-Linked
Oligosaccharides from Glycoproteins and Glycopeptides
Abstract | Full Text:
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Supplement 30,
April 1995
Unit 7.7 Computer Manipulation of DNA and Protein
Sequences
Abstract | Full Text:
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PDF
Unit 15.1 Enzymatic Amplification of DNA by PCR: Standard
Procedures and Optimization
Unit 18.1 Overview of Protein Phosphorylation
Unit 18.2 Labeling Cultured Cells with 32 P
i and Preparing Cell Lysates for
Immunoprecipitation
Abstract | Full Text:
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PDF
Unit 18.3 Phosphoamino Acid Analysis
Abstract | Full Text:
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PDF
Unit 18.4 Analysis of Phosphorylation of Unlabeled
Proteins
Abstract | Full Text:
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PDF
Unit 19.1 Internet Basics for Biologists
Unit 19.2 Sequence Databases: Information Retrieval and Data
Submission
[
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Supplement 29,
January 1995
Unit 5.5 Conversion of mRNA into Double-Stranded
cDNA
Abstract | Full Text:
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PDF
Unit 9.6 Overview of Genetic Reporter Systems
Abstract | Full Text:
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PDF
Unit 9.7B Nonisotopic Assays for Reporter Gene
Activity
Abstract | Full Text:
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PDF
Unit 17.15A β-Elimination for Release of O -Linked
Glycosaminoglycans from Proteoglycans
Abstract | Full Text:
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PDF
Appendix 1 Safe Use of Radioisotopes
Appendix 5 Vectors
Abstract | Full Text:
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PDF
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Supplement 28,
October 1994
Unit 16.4A Introduction to Expression by Fusion Protein
Vectors
Abstract | Full Text:
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PDF
Unit 16.4B Enzymatic and Chemical Cleavage of Fusion
Proteins
Abstract | Full Text:
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PDF
Unit 16.8 Expression and Purification of Thioredoxin Fusion
Proteins
Abstract | Full Text:
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PDF
Unit 16.10 Preparation of Insect Cell Cultures and Baculovirus
Stock
Unit 16.11 Generation of Recombinant Baculoviruses and Analysis
of Recombinant Protein Expression
Appendix 3A Detection and Quantitation of Radiolabeled Proteins
and DNA in Gels and Blots
Appendix 3B Silanizing Glassware
Abstract | Full Text:
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PDF
Appendix 3C Dialysis and Ultrafiltration
Abstract | Full Text:
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PDF
Appendix 3D Quantitation of DNA and RNA with Absorption and
Fluorescence Spectroscopy
Appendix 3E Introduction of Restriction Enzyme Recognition
Sequences by Silent Mutation
Abstract | Full Text:
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PDF
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Supplement 27,
July 1994
Unit 1.7 Large-Scale Preparation of Plasmid DNA
Unit 2.3 Preparation of Genomic DNA from Plant Tissue
Abstract | Full Text:
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PDF
Unit 6.12 Recombination-Based Assay (RBA) for Screening
Bacteriophage Lambda Libraries
Abstract | Full Text:
HTML
PDF
Unit 13.14 Interaction Trap/Two-Hybrid System to Identify
Interacting Proteins
Unit 17.13B Analysis of Glycosaminoglycans with Polysaccharide
Lyases
Abstract | Full Text:
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PDF
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Supplement 26,
April 1994
Unit 2.6 Isolation and Purification of Large DNA Restriction
Fragments from Agarose Gels
Unit 4.10 Identification of Newly Transcribed RNA
Full Text:
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PDF
Unit 7.4B Dideoxy DNA Sequencing with Chemiluminescent
Detection
Unit 15.8 Differential Display of mRNA by CPR
Unit 17.5 Chemical Labeling of Carbohydrates by Oxidation and
Sodium Borohydride Reduction
Abstract | Full Text:
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PDF
[
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Supplement 25,
January 1994
Unit 2.1 Purification of DNA Using Glass Beads
Unit 2.7 Separation of Small DNA Fragments by Conventional Gel
Electrophoresis
Unit 12.3 Methylation and Uracil Interference Assays for
Analysis of Protein-DNA Interactions
Abstract | Full Text:
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PDF
Unit 16.20 Expression of Proteins Using Semliki Forest Virus
Vectors
Abstract | Full Text:
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PDF
[
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Supplement 24,
October 1993
Unit 2.14 Purification of DNA Using Anion Exchange
Chromatography
Unit 3.17 Constructing Recombinant DNA Molecules by the
Polymerase Chain Reaction
Full Text:
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PDF
Unit 10.11B Metal-Chelate Affinity Chromatography
Unit 12.10 Purification of Sequence-Specific DNA-Binding
Proteins by Affinity Chromatography
Abstract | Full Text:
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PDF
Unit 13.11 Preparation of Yeast DNA
Abstract | Full Text:
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PDF
[
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Supplement 23,
July 1993
Unit 4.9 Analysis of RNA by Northern and Slot Blot
Hybridization
Unit 6.11 Use of Monoclonal Antibodies for Expression
Cloning
Abstract | Full Text:
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PDF
Unit 9.15 Overview of Gene Targeting by Homologous
Recombination
Unit 9.16 Production of Heterozygous Mutant Cell Line by
Homologous Recombination (Single Knockout)
Unit 9.17 Production of Homozygous Mutant Embryonic Stem Cell
Line (Double Knockout)
Unit 13.12 Preparation of Yeast RNA
Abstract | Full Text:
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PDF
Unit 17.7 Lectin Analysis of Proteins Blotted onto
Filters
Abstract | Full Text:
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PDF
Unit 17.14 Preparation of Glycopeptides
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Supplement 22,
April 1993
Unit 17.1 Special Considerations for Glycoproteins and Their
Purification
Abstract | Full Text:
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PDF
Unit 17.2 Special Considerations for Proteoglycans and
Glycosaminoglycans and Their Purification
Abstract | Full Text:
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PDF
Unit 17.3 Special Considerations for Glycolipids and Their
Purification
Abstract | Full Text:
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PDF
Unit 17.4 Metabolic Radiolabeling of Animal Cell
Glycoconjugates
Full Text:
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PDF
Unit 17.6 Analysis of Saccharide Structure and Function Using
Glycotransferases
Unit 17.8 Detection of Glycophospholipid Anchors on
Proteins
Abstract | Full Text:
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PDF
Unit 17.9 Phenol-Sulfuric Acid Assay for Hexoses and
Pentoses
Unit 17.10 Inhibition of N-Linked Glycosylation
Unit 17.12 Sialidases
Abstract | Full Text:
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PDF
Unit 17.13A Endoglycosidase and Glycoamidase Release of N-Linked
Oligosaccharides
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Page]
Supplement 21,
January 1993
Unit 2.9A Southern Blotting
Abstract | Full Text:
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PDF
Unit 2.9B Dot and Slot Blotting of DNA
Abstract | Full Text:
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PDF
Unit 2.10 Hybridization Analysis of DNA Blots
Abstract | Full Text:
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PDF
Unit 7.3 Preparation of Templates for DNA Sequencing
Abstract | Full Text:
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PDF
Unit 7.4 DNA Sequencing by the Dideoxy Method
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Supplement 20,
October 1992
Unit 3.18 Labeling and Colorimetric Detection of Nonisotopic
Probes
Abstract | Full Text:
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PDF
Unit 3.19 Chemiluminescent Detection of Nonisotopic
Probes
Abstract | Full Text:
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PDF
Unit 4.8 Primer Extension
Abstract | Full Text:
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PDF
Unit 6.9 Overview of Strategies for Screening YAC Libraries and
Analyzing YAC Clones
Abstract | Full Text:
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PDF
Unit 6.10 Analysis of Isolated YAC Clones
Abstract | Full Text:
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PDF
Unit 15.5 Ligand-Mediated PCR for Genomic Sequencing and
Footprinting
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Supplement 19,
July 1992
Unit 3.1 Digestion of DNA with Restriction
Endonucleases
Abstract | Full Text:
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PDF
Unit 7.7 Computer Analysis of DNA and and Protein
Sequences
Unit 10.19 Isolation of Proteins for Microsequence
Analysis
Abstract | Full Text:
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PDF
Unit 13.2 Growth and Manipulation of Yeast
Full Text:
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PDF
Unit 15.7 Molecular Cloning of PCR Products
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Supplement 18,
April 1992
Unit 5.8A Production of a Complete cDNA Library
Abstract | Full Text:
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PDF
Unit 10.4 Two-Dimensional Gel Electrophoresis Using the
O'Farrell System
Full Text:
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PDF
Unit 10.8 Immunoblotting and Immunodetection
Unit 11.3 Isotype Determination of Antibodies
Abstract | Full Text:
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PDF
Unit 11.4 Immunization of Mice
Abstract | Full Text:
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PDF
Unit 11.10 Production of Monoclonal Antibody Supernatant and
Ascites Fluid
Full Text:
HTML
PDF
Unit 13.7 Introduction of DNA into Yeast Cells
Abstract | Full Text:
HTML
PDF
Unit 16.14 Amplification Using CHO Cell Expression
Vectors
Unit 16.19 Gene Expression Using the Vaccinia Virus/T7 RNA
Polymerase Hybrid System
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Page]
Supplement 17,
January 1992
Unit 7.5 DNA Sequencing by the Chemical Method
Abstract | Full Text:
HTML
PDF
Unit 9.10 Overview of the Retrovirus Transduction
System
Unit 9.11 Preparation of a Specific Retrovirus Producer Cell
Line
Unit 9.12 Large-Scale Preparation and Concentration of
Retrovirus Stocks
Unit 9.13 Detection of Helper Virus in Retrovirus
Stocks
Unit 9.14 Retrovirus Infection of Cells In Vitro and In
Vivo
Unit 15.6 cDNA Amplification Using One-Sided (Anchored)
PCR
Abstract | Full Text:
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PDF
[
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Supplement 16,
October 1991
Unit 7.1 DNA Sequencing Strategies
Unit 7.2 Constructing Nested Deletions for Use in DNA
Sequencing
Abstract | Full Text:
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PDF
Unit 7.3 Preparation of Templates for DNA Sequencing
Unit 7.4 DNA Sequencing by the Dideoxy Method
Unit 7.6 Denaturing Gel Electrophoresis for Sequencing
Abstract | Full Text:
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PDF
Unit 15.2 Direct DNA Sequencing of PCR Products
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Supplement 15,
July 1991
Unit 1.6 Minipreps of Plasmid DNA
Abstract | Full Text:
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PDF
Unit 4.4 Preparation of Bacterial RNA
Abstract | Full Text:
HTML
PDF
Unit 8.5 Mutagenesis by the Polymerase Chain Reaction
Unit 16.15 Overview of the Vaccinia Virus Expression
System
Unit 16.16 Preparation of Cell Cultures and Vaccinia Virus
Stocks
Unit 16.17 Generation of Recombinant Vaccinia Viruses
Unit 16.18 Characterization of Recombinant Vaccinia Viruses and
Their Products
[
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Page]
Supplement 14,
April 1991
Unit 4.2 Guanidine Methods for Total RNA
Preparation
Unit 9.4 Liposome-Mediated Transfection
Unit 15.5 Ligation-Mediated PCR for Genomic Sequencing and
Footprinting
Unit 16.12 Overview of Protein Expression in Mammalian
Cells
Unit 16.13 Transient Expression of Proteins Using COS
Cells
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Supplement 13,
January 1991
Unit 5.6 Ligation of Linkers or Adapters to Double-Stranded
cDNA
Abstract | Full Text:
HTML
PDF
Unit 10.2 One-Dimensional Gel Electrophoresis of
Proteins
Unit 10.6 Staining Proteins in Gels
Unit 10.18 Biosynthetic Labeling of Proteins
Unit 12.7 Detection, Purification, and Characterization of cDNA
Clones Encoding DNA-Binding Proteins
Abstract | Full Text:
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PDF
Unit 14.5 Counterstaining and Mounting of Autoradiographed In
Situ Hybridization Slides
Abstract | Full Text:
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PDF
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Supplement 12,
October 1990
Unit 3.18 Nonisotopic Labeling of DNA Using Nick
Translation
Unit 9.6 Reporter System Using Firefly Luciferase
Unit 13.3 Mutagenesis of Yeast Cells
Appendix 1 Characteristics of Amino Acids
Appendix 1 Characteristics of Nucleic Acids
[
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Supplement 11,
July 1990
Unit 3.1 Digestion of DNA with Restriction
Endonucleases
Unit 9.1 Calcium Phosphate Transfection
Unit 12.1 Preparation of Nuclear and Cytoplasmic Extracts from
Mammalian Cells
Full Text:
HTML
PDF
Unit 16.1 Overview of Protein Expression in E. coli
Abstract | Full Text:
HTML
PDF
Unit 16.2 Expression Using the T7 RNA Polymerase/Promoter
System
Abstract | Full Text:
HTML
PDF
Unit 16.3 Expression Using Vectors with Phage Regulatory
Sequences
Abstract | Full Text:
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PDF
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Supplement 10,
April 1990
Unit 1.13 Preparing Lambda DNA from Phage Lysates
Abstract | Full Text:
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PDF
Unit 11.1 Conjugation of Enzymes to Antibodies
Abstract | Full Text:
HTML
PDF
Unit 11.2 Enzyme-Linked Immunosorbent Assays (ELISA) for
Detection of Antigens
Abstract | Full Text:
HTML
PDF
Unit 16.4 Introduction to Expression by Fusion Protein
Vectors
Unit 16.5 Expression and Purification of lacZ and trpE Fusion
Proteins
Abstract | Full Text:
HTML
PDF
Unit 16.6 Expression and Purification of Maltose-Binding Protein
Fusions
Abstract | Full Text:
HTML
PDF
Unit 16.7 Expression and Purification of Glutathione- S
-Transferase Fusion Proteins
Abstract | Full Text:
HTML
PDF
Unit 16.8 Overview of the Baculoviral Expression
System
Unit 16.9 Preparation of Insect Cell Cultures and Baculovirus
Stock
Unit 16.10 Purification and Transfection of Baculoviral DNA for
Generating Recombinant Viruses
Unit 16.11 Generation of Recombinant Baculoviruses and Analysis
of Recombinant Protein Expression
[
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Supplement 9,
January 1990
Unit 2.9 Electroblotting and UV Crosslinking of DNA
Unit 3.5 DNA-Dependent DNA Polymerases
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Unit 4.10 Identification of Newly Transcribed RNA
Unit 9.6 Phase-Extraction Assay for CAT Activity
Unit 13.13 Preparation of Protein Extracts from Yeast
Abstract | Full Text:
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Unit 15.1 Enzymatic Amplification of DNA by Polymerase Chain
Reaction: Standard Procedures and Optimization
Unit 15.2 Direct DNA Sequencing of Polymerase Chain Reaction
Products
Unit 15.3 Quantitation of Rare DNAs by the Polymerase Chain
Reaction
Unit 15.4 Enzymatic Amplification of RNA by the Polymerase Chain
Reaction
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Supplement 8,
October 1989
Unit 1.7 Large-Scale Preparation of Plasmid DNA
Unit 1.8 Introduction of Plasmid DNA into Cells
Unit 2.11 Synthesis of Oligonucleotides
Unit 3.13 Ribonucleases
Abstract | Full Text:
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Unit 10.17 Synthesizing Proteins In Vitro by Transcription and
Translation of Cloned Genes
Abstract | Full Text:
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Unit 12.9 Analysis of DNA-Protein Interactions Using Proteins
Synthesized In Vitro from Cloned Genes
Abstract | Full Text:
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Appendix 3 Commonly Used Biochemical Techniques
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Supplement 7, July
1989
Unit 12.4 DNase I Footprint Analysis of Protein-DNA
Binding
Abstract | Full Text:
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Unit 14.1 Fixation, Embedding, and Sectioning of Tissues,
Embryos, and Single Cells
Abstract | Full Text:
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Unit 14.2 Cryosectioning
Abstract | Full Text:
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Unit 14.3 In Situ Hybridization to Cellular RNA
Unit 14.4 Detection of Hybridized Probe
Abstract | Full Text:
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Unit 14.5 Counterstaining and Mounting of Autoradiographed In
Situ Hybridization Slides
Unit 14.6 Immunohistochemistry
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Supplement 6,
April 1989
Unit 2.9 Southern Blotting and Hybridization
Unit 2.12 Purification of Oligonucleotides Using Denaturing
Polyacrylamide Gel Electrophoresis
Unit 8.2B Gene Synthesis: Assembly of Target Sequences Using
Mutually Priming Long Oligonucleotides
Abstract | Full Text:
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Unit 11.12 Production of Polyclonal Antisera in
Rabbits
Unit 11.13 Purification of Immunoglobin G Fraction from
Antiserum, Ascites Fluid, or Hydridoma Supernatant
Unit 11.14 Selection of an Immugenic Peptide
Unit 11.15 Production of Antipeptide Antibodies
Unit 11.16 Determination of the Specific Antibody
Titer
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Supplement 5,
January 1989
Unit 1.8 Introduction of Plasmid DNA into Cells
Unit 3.1 Digestion of DNA with Restriction
Endonucleases
Unit 13.1 Preparation of Yeast Media
Abstract | Full Text:
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Unit 13.2 Growth and Manipulation of Yeast
Unit 13.4 Yeast Cloning Vectors and Genes
Abstract | Full Text:
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Unit 13.6 Yeast Cloning Vectors and Genes
Unit 13.7 Introduction of DNA into Yeast Cells
Unit 13.8 Cloning Yeast Genes by Complementation
Abstract | Full Text:
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Unit 13.9 Segregation of Plasmids from Yeast Cells
Unit 13.10 Manipulation of Cloned Yeast DNA
Unit 13.11 Preparation of Yeast DNA
Unit 13.12 Preparation of Yeast RNA
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Supplement 4,
October 1988
Unit 2.5A Agarose Gel Electrophoresis
Unit 2.5B Pulsed-Field Gel Electrophoresis
Unit 2.10 Separation of Double- and Single-Stranded Nucleic
Acids Using Hydroxylapatite Chromatography
Unit 3.5 DNA-Dependent DNA Polymerases
Unit 3.17 The Polymerase Chain Reaction: In Vitro Enzymatic
Amplification of DNA
Unit 5.8A Production of a Completed cDNA Library
Unit 5.8B Production of a Subtracted cDNA
Library
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Supplement 3, July
1988
Unit 12.2 Mobility Shift DNA-Binding Assay Using Gel
Electrophoresis
Unit 12.3 Methylation Interference Assay for Analysis of
DNA-Protein Interactions
Unit 12.5 UV Crosslinking of Proteins to Nucleic Acids
Abstract | Full Text:
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Unit 12.6 Purification of DNA-Binding Proteins Using
Biotin/Streptavidin Affinity Systems
Abstract | Full Text:
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Unit 12.7 Detection, Purification, and Characterization of cDNA
Clones Encoding DNA-Binding Proteins
Unit 12.8 Rapid Separation of Protein-Bound DNA from Free DNA
Using Nitrocellulose Filters
Abstract | Full Text:
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Supplement 2,
April 1988
Unit 6.4 Using Synthetic Oligonucleotides as Probes
Abstract | Full Text:
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Unit 7.3 Construction of Nested Deletions for DNA
Sequencing
Unit 7.4 Sequencing by the Dideoxy Method
Unit 8.4 Linker-Scanning Mutagenesis of DNA
Abstract | Full Text:
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Unit 10.15 High-Performance Chromatofocusing and
Hydrophobic-Interaction Chromatography
Appendix 1 Standard Measurements, Data, and
Abbreviations
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Supplement 1,
January 1988
Unit 4.1 Preparation of Cytoplasmic RNA from Tissue Culture
Cells
Unit 4.6 S1 Analysis of Messenger RNA Using Single-Stranded DNA
Probes
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Unit 11.1 Conjugation of Enzymes to Antibodies
Unit 11.2 Enzyme-Linked Immunosorbent Assays (ELISA) for
Detection of Antigens
Unit 11.3 Immunization of Mice
Unit 11.4 Direct Enzyme-Linked Immunosorbent Assay (ELISA) for
Detection of Antibodies in Serum and Hybridoma
Supernatants
Unit 11.5 Preparation of Myeloma Cells
Abstract | Full Text:
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Unit 11.6 Preparation of Mouse Feeder Cells for Fusion and
Cloning
Abstract | Full Text:
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Unit 11.7 Fusion of Myeloma Cells with Immune Spleen
Cells
Abstract | Full Text:
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Unit 11.8 Cloning of Hybridoma Cell Lines by Limiting
Dilution
Abstract | Full Text:
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Unit 11.9 Freezing and Recovery of Hybridoma Cell
Lines
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Unit 11.10 Production of Ascites Fluid
Unit 11.11 Purification of Monoclonal Antibodies
Abstract | Full Text:
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Appendix 3 Commonly Used Biochemical Techniques
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Core, 1987
Unit 1.1 Media Preparation and Bacteriological
Tools
Unit 1.2 Growth in Liquid Media
Unit 1.3 Growth on Solid Media
Unit 1.4 Selected Topics from Classical Bacterial
Genetics
Unit 1.5 Introduction to Plasmid Biology
Unit 1.6 Minipreps of Plasmid DNA
Unit 1.7 CsCl/Ethidium Bromide Preparations of Plasmid
DNA
Unit 1.8 Introduction of Plasmid DNA into Cells
Unit 1.9 Introduction to Lambda Phages
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Unit 1.10 Lambda as a Cloning Vector
Abstract | Full Text:
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Unit 1.11 Plating Lambda Phage to Generate Plaques
Abstract | Full Text:
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Unit 1.12 Growing Lambda-Derived Vectors
Abstract | Full Text:
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Unit 1.13 Making Phage DNA from Liquid Lysates
Unit 1.14 Introduction to Vectors Derived from Filamentous
Phages
Abstract | Full Text:
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Unit 1.15 Preparing and Using M13-Derived Vectors
Abstract | Full Text:
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Unit 2.1 Purification and Concentration of DNA from Aqueous
Solutions
Unit 2.2 Preparation of Genomic DNA from Mammalian
Tissue
Abstract | Full Text:
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Unit 2.3 Preparation of Genomic DNA from Plant Tissue
Unit 2.4 Preparation of Genomic DNA from Bacteria
Abstract | Full Text:
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Unit 2.5 Agarose Gel Electrophoresis
Unit 2.6 Isolation and Purification of Large DNA Restriction
Fragments from Agarose Gels
Unit 2.7 Nondenaturing Polyacrylamide Gel
Electrophoresis
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Unit 2.8 Sieving Agarose Gel Electrophoresis
Unit 2.9 Southern Blotting and Hybridization
Unit 3.1 Digestion of DNA with Restriction
Endonucleases
Unit 3.2 Mapping by Multiple Endonuclease Digestions
Abstract | Full Text:
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Unit 3.3 Mapping by Partial Endonuclease Digestions
Abstract | Full Text:
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Unit 3.4 Reagents and Radioisotopes Used to Manipulate Nucleic
Acids
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Unit 3.5 DNA-Dependent DNA Polymerases
Unit 3.6 Template-Independent DNA Polymerases
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Unit 3.7 RNA-Dependent DNA Polymerases
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Unit 3.8 DNA-Dependent RNA Polymerases
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Unit 3.9 DNA-Independent RNA Polymerases
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Unit 3.10 Phosphatases and Kinases
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Unit 3.11 Exonucleases
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Unit 3.12 Endonucleases
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Unit 3.13 Nonspecific Double-Stranded Endonucleases
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Unit 3.14 DNA Ligases
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Unit 3.15 RNA Ligases
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Unit 3.16 Subcloning of DNA Fragments
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Unit 4.1 Preparation of Cytoplasmic RNA from Tissue Culture
Cells
Unit 4.2 Guanidine Methods for Total RNA Preparation
Unit 4.3 Phenol/ SDS Method for Plant RNA Preparation
Abstract | Full Text:
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Unit 4.4 Preparation of Bacterial RNA
Unit 4.5 Preparation of Poly(A) RNA
Abstract | Full Text:
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Unit 4.6 S1 Analysis of Messenger RNA Using Single-Stranded DNA
Probes
Unit 4.7 Ribonuclease Protection Assay
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Unit 4.8 Primer Extension
Unit 4.9 Analysis of RNA by Northern Hybridization
Unit 4.10 Identification of Newly Transcribed RNA
Unit 5.1 Genomic DNA Libraries
Abstract | Full Text:
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Unit 5.2 cDNA Libraries
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Unit 5.3 Size Fractionation Using Sucrose Gradients
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Unit 5.4 Size Fractionation Using Agarose Gels
Abstract | Full Text:
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Unit 5.5 Conversion of mRNA into Double-Stranded cDNA
Unit 5.6 Methylation and Addition of Linkers to Double-Stranded
cDNA
Unit 5.7 Production of a Genomic Library
Abstract | Full Text:
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Unit 5.8 Production of a cDNA Library
Unit 5.9 Amplification of a Bacteriophage Lambda
Library
Unit 5.10 Amplification of Cosmid and Plasmid
Libraries
Unit 6.1 Plating and Transferring Bacteriophage
Libraries
Abstract | Full Text:
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Unit 6.2 Plating and Transferring Cosmid and Plasmid
Libraries
Abstract | Full Text:
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Unit 6.3 Using DNA Fragments as Probes
Abstract | Full Text:
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Unit 6.4 Using Synthetic Oligonucleotides as Probes
Unit 6.5 Purification of Bacteriophage Clones
Abstract | Full Text:
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Unit 6.6 Purification of Cosmid and Plasmid Clones
Abstract | Full Text:
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Unit 6.7 Immunoscreening of Fusion Proteins Produced in Lambda
Plaques
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Unit 6.8 Immunoscreening after Hybrid Selection and
Translation
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Unit 7.1 Overview of DNA Sequencing Strategies
Unit 7.2 Computer-Assisted DNA Sequence Data Entry and DNA
Sequence Manipulation
Unit 7.3 Construction of Nested Deletions for DNA Sequencing
Using Bal 31 Exonuclease
Unit 7.4 Sequencing by the Dideoxy Method
Unit 7.5 Sequencing by the Chemical Method
Unit 7.6 Rapid DNA Sequencing by the Chemical Degradation Method
Utilizing Specialized Vectors for Cloning Nested
Deletions
Unit 8.1 Oligonucleotide-Directed Mutagenesis without Phenotypic
Selection
Abstract | Full Text:
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Unit 8.2 Mutagenesis with Degenerate Oligonucleotides: Creating
Numerous Mutations in a Small DNA Sequence
Unit 8.3 Region-Specific Mutagenesis
Unit 9.1 Calcium Phosphate Transfection
Unit 9.2 Transfection Using DEAE-Dextran
Unit 9.3 Transfection by Electroporation
Abstract | Full Text:
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Unit 9.4 Optimization of Transfection
Unit 9.5 Stable Transfer of Genes into Mammalian Cells
Unit 9.6 Harvest and Assay for Chloramphenicol
Acetyltransferase
Unit 9.7 Assay for Human Growth Hormone
Unit 9.8 Direct Analysis of RNA after Transfection
Abstract | Full Text:
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Unit 9.9 Amplification Using Dihydrofolate Reductase
Unit 10.1 Colorimetric Methods
Unit 10.2 One-Dimensional Gel Electrophoresis
Unit 10.3 Two-Dimensional Gel Electrophoresis Using the ISO-DALT
System
Abstract | Full Text:
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Unit 10.4 Two-Dimensional Gel Electrophoresis Using the
O'Farrell System
Unit 10.5 Electroelution of Proteins from Stained Gels
Unit 10.6 Detection of Protein in Gels
Unit 10.7 Detection of Proteins on Blot Transfer
Membranes
Unit 10.8 Western Blotting
Unit 10.9 Gel-Filtration Chromatography
Unit 10.10 Ion-Exchange Chromatography
Unit 10.11 Immunoaffinity Chromatography
Unit 10.12 Reversed-Phase High-Performance Liquid
Chromatography
Unit 10.13 Ion-Exchange High-Performance Liquid
Chromatography
Unit 10.14 Size-Exclusion High-Performance Liquid
Chromatography
Unit 10.15 High-Performance Hydrophobic-Interaction
Chromatography
Unit 10.16 Immunoprecipitation
Appendix 1 Standard Abbreviations and Useful Data
Appendix 2 Stock Solutions and Buffers
Appendix 3 Commonly Used Biochemical Techniques
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