Archive

 

Current Protocols in Cell Biology Supplements

2009:    Supplement 45     Supplement 44     Supplement 43     Supplement 42
2008:     Supplement 41     Supplement 40     Supplement 39     Supplement 38
2007:    Supplement 37     Supplement 36     Supplement 35     Supplement 34    
2006:    Supplement 33     Supplement 32     Supplement 31     Supplement 30    
2005:    Supplement 29     Supplement 28     Supplement 27     Supplement 26    
2004:    Supplement 25     Supplement 24     Supplement 23     Supplement 22    
2003:    Supplement 21     Supplement 20     Supplement 19     Supplement 18    
             Supplement 17    
2002:    Supplement 16     Supplement 15     Supplement 14     Supplement 13    
2001:    Supplement 12     Supplement 11     Supplement 10     Supplement 9      
2000:    Supplement 8       Supplement 7       Supplement 6       Supplement 5      
1999:    Supplement 4       Supplement 3       Supplement 2       Supplement 1      
1998:    Core publication

  Supplement 45, December 2009

Unit 4.23 Interference Reflection Microscopy
         Abstract | Full Text:  HTML   PDF

Unit 4.24 Fluorescence Correlation Spectroscopy in Living Cells: A Practical Approach
         Abstract | Full Text:  HTML   PDF

Unit 10.15 Generation of Micropatterned Substrates Using Micro Photopatterning
         Abstract | Full Text:  HTML   PDF

Unit 14.10 In Vivo Imaging of Signal Transduction Cascades with Probes Based on Förster Resonance Energy Transfer (FRET)
         Abstract | Full Text:  HTML   PDF

  Supplement 44, September 2009

Unit 3.33 Isolation and Biochemical Characterization of Amyloid Plaques and Paired Helical Filaments
         Abstract | Full Text:  HTML   PDF

Unit 19.12 Overview: Generation of Gene Knockout Mices
         Abstract | Full Text:  HTML   PDF

Unit 19.13 Manipulation of Mouse Embryonic Stem Cells for Knockout Mouse Production
         Abstract | Full Text:  HTML   PDF

Unit 19.14 Generation of Gene Knockout Mice by ES Cell Microinjection
         Abstract | Full Text:  HTML   PDF

  Supplement 43, June 2009

Unit 4.22 Culturing MDCK Cells in Three Dimensions for Analyzing Intracellular Dynamics
         Abstract | Full Text:  HTML   PDF

Unit 14.9 In Vitro GEF and GAP Assays
         Abstract | Full Text:  HTML   PDF

Unit 26.3 Methods Used to Study Respiratory Virus Infection
         Abstract | Full Text:  HTML   PDF

Unit 26.4 Compartmented Neuron Cultures for Directional Infection by Alpha Herpesviruses
         Abstract | Full Text:  HTML   PDF

Unit 26.5 HIV-1 Interactions with Cells: From Viral Binding to Cell-Cell Transmission
         Abstract | Full Text:  HTML   PDF

  Supplement 42, March 2009

Unit 3.32 Isolation of Dense Core Secretory Vesicles from Pancreatic Endocrine Cells by Differential and Density Gradient Centrifugation
         Abstract | Full Text:  HTML   PDF

Unit 19.10 Overview: Engineering Transgenic Constructs and Mice
         Abstract | Full Text:  HTML   PDF

Unit 19.11 Generation of Transgenic Mice
         Abstract | Full Text:  HTML   PDF

Unit 24.4 Visualization of Cellular Phosphoinositide Pools with GFP-Fused Protein-Domains
         Abstract | Full Text:  HTML   PDF

Unit 25.2 Fabrication and Application of Nanofibrous Scaffolds in Tissue Engineering
         Abstract | Full Text:  HTML   PDF

Unit 26.2 BK Virus (BKV): Infection, Propagation, Quantitation, Purification, Labeling, and Analysis of Cell Entry
         Abstract | Full Text:  HTML   PDF

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  Supplement 41, December 2008

Unit 3.31 Isolation of Neuromelanin Granules
         Abstract | Full Text:  HTML   PDF

Unit 4.21 Photoactivated Localization Microscopy (PALM) of Adhesion Complexes
         Abstract | Full Text:  HTML   PDF

Unit 19.8 Embryonic Organ Culture
         Abstract | Full Text:  HTML   PDF

Unit 19.9 Three-Dimensional Tissue Models of Normal and Diseased Skin
         Abstract | Full Text:  HTML   PDF

Unit 22.13 Monitoring mRNA Export
         Abstract | Full Text:  HTML   PDF

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  Supplement 40, September 2008

Unit 3.30 Purification of Intact Chloroplasts from Arabidopsis and Spinach Leaves by Isopycnic Centrifugation
         Abstract | Full Text:  HTML   PDF

Unit 10.8 Matrix Metalloproteinases
         Abstract | Full Text:  HTML   PDF

Unit 10.14 Use of Hyaluronan-Derived Hydrogels for Three-Dimensional Cell Culture and Tumor Xenografts
         Abstract | Full Text:  HTML   PDF

Unit 15.13 Analysis of Endocytic Trafficking by Single-Cell Fluorescence Ratio Imaging
         Abstract | Full Text:  HTML   PDF

Unit 24.3 Making Giant Unilamellar Vesicles via Hydration of a Lipid Film
         Abstract | Full Text:  HTML   PDF

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  Supplement 39, June 2008

Unit 3.29 Isolation of Microtubules and Microtubule Proteins
         Abstract | Full Text:  HTML   PDF

Unit 4.18 Fluorescence Imaging Techniques for Studying Drosophila Embryo Development
         Abstract | Full Text:  HTML   PDF

Unit 4.19 Quantitative Colocalization Analysis of Confocal Fluorescence Microscopy Images
         Abstract | Full Text:  HTML   PDF

Unit 4.20 Visualizing Protease Activity in Living Cells: From Two Dimensions to Four Dimensions
         Abstract | Full Text:  HTML   PDF

Unit 22.11 Assays for Ribosomal RNA Processing and Ribosome Assembly
         Abstract | Full Text:  HTML   PDF

Unit 22.12 Visualization and Measurement of DNA Methyltransferase Activity in Living Cells
         Abstract | Full Text:  HTML   PDF

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  Supplement 38, March 2008

Unit 3.28 Isolation of Amyloplasts
         Abstract | Full Text:  HTML   PDF (311K)

Unit 6.10 Two-Dimensional Blue Native Polyacrylamide Gel Electrophoresis
         Abstract | Full Text:  HTML   PDF (301K)

Unit 14.8 Rho GTPase Activation Assays
         Abstract | Full Text:  HTML   PDF (319K)

Unit 21.6 Photoactivation and Imaging of Photoactivatable Fluorescent Proteins
         Abstract | Full Text:  HTML   PDF (445K)

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  Supplement 37, December 2007

Unit 3.27 Isolation of Endoplasmic Reticulum, Mitochondria, and Mitochondria-Associated Membrane Fractions from Transfected Cells and from Human Cytomegalovirus-Infected Primary Fibroblasts
         Abstract | Full Text:  HTML   PDF (655K)

Unit 4.17 Scanning Electron Microscopy of Cell Surface Morphology
         Abstract | Full Text:  HTML   PDF (404K)

Unit 6.1 One-Dimensional SDS Gel Electrophoresis of Proteins
         Abstract | Full Text:  HTML   PDF (891K)

Unit 17.1 Visualization of RNA Using Fluorescence Complementation Triggered byAptamer-Protein Interactions (RFAP) in Live Bacterial Cells
         Abstract | Full Text:  HTML   PDF (466K)

Unit 26.1 Production of Papillomavirus-Based Gene Transfer Vectors
         Abstract | Full Text:  HTML   PDF (294K)

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  Supplement 36, September 2007

Unit 1.1 Basic Techniques in Mammalian Cell Tissue Culture
         Abstract | Full Text:  HTML   PDF (205K)

Unit 15.12 Analysis of Regulated Secretion Using PC12 Cells
         Abstract | Full Text:  HTML   PDF (329K)

Unit 23.6 Hematoendothelial Differentiation of Human Embryonic Stem Cells
         Abstract | Full Text:  HTML   PDF (507K)

Unit 23.7 Neural Differentiation of Human ES Cells
         Abstract | Full Text:  HTML   PDF (249K)

Unit 25.1 In Vivo Imaging Using Quantum Dot–Conjugated Probes
         Abstract | Full Text:  HTML   PDF (409K)

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  Supplement 35, June 2007

Unit 4.16 Analyzing Real-Time Video Microscopy: The Dynamics and Geometry of Vesicles and Tubules in Endocytosis
         Abstract | Full Text:  HTML   PDF (378K)

Unit 19.7 Imaging Tumor Cell Movement In Vivo
         Abstract | Full Text:  HTML   PDF (316K)

Unit 22.10 Replication Labeling with Halogenated Thymidine Analogs
         Abstract | Full Text:  HTML   PDF (197K)

Unit 24.1 Using Fluorescent Sphingolipid Analogs to Study Intracellular Lipid Trafficking
         Abstract | Full Text:  HTML   PDF (725K)

Unit 24.2 Fluorescent Detection of Lipid Droplets and Associated Proteins
         Abstract | Full Text:  HTML   PDF (174K)

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  Supplement 34, March 2007

Unit 3.26 Isolation of Renal Brush Borders
         Abstract | Full Text:  HTML   PDF (610K)

Unit 4.15 Biological Second and Third Harmonic Generation Microscopy
         Abstract | Full Text:  HTML   PDF (1435K)

Unit 11.19 In Vitro Analysis of Yeast Mitochondrial Protein Import
         Abstract | Full Text:  HTML   PDF (154K)

Unit 23.3 Maintenance and In Vitro Differentiation of Mouse Embryonic Stem Cells to Form Blood Vessels
         Abstract | Full Text:  HTML   PDF (350K)

Unit 23.4 Differentiation of Mouse Embryonic Stem Cells and of Human Adult Stem Cells into Adipocytes
         Abstract | Full Text:  HTML   PDF (422K)

Unit 23.5 Induction of ES Cell–Derived Cartilage Formation
         Abstract | Full Text:  HTML   PDF (684K)

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  Supplement 33, December 2006

Unit 3.25 Isolation of Myelin
         Abstract | Full Text:  HTML   PDF (437K)

Unit 10.9 Preparation of Extracellular Matrices Produced by Cultured Fibroblasts
         Abstract | Full Text:  HTML   PDF (389K)

Unit 17.10 Identification and Analysis of Multiprotein Complexes Through Chemical Crosslinking
         Abstract | Full Text:  HTML   PDF (891K)

Unit 21.5 The Fluorescent Protein Color Palette
         Abstract | Full Text:  HTML   PDF (1079K)

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  Supplement 32, September 2006

Unit 2.7 Preparation and Coculture of Neurons and Glial Cells
         Abstract | Full Text:  HTML   PDF (212K)

Unit 15.10 Measuring Retrograde Transport to the Trans -Golgi Network
         Abstract | Full Text:  HTML   PDF (334K)

Unit 15.11 Assays for Regulated Exocytosis of Mast Cell Granules
         Abstract | Full Text:  HTML   PDF (159K)

Unit 17.9 Rational Design and Evaluation of FRET Experiments to Measure Protein Proximities in Cells
         Abstract | Full Text:  HTML   PDF (844K)

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  Supplement 31, June 2006

Unit 3.23 Isolation of Intermediate Filaments
         Abstract | Full Text:  HTML   PDF (161K)

Unit 3.24 Isolation of T-Tubules from Skeletal Muscle
         Abstract | Full Text:  HTML   PDF (496K)

Unit 15.9 Studies of the Ubiquitin Proteasome System
         Abstract | Full Text:  HTML   PDF (622K)

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  Supplement 30, March 2006

Unit 3.20 Isolation of GLUT4 Storage Vesicles
         Abstract | Full Text:  HTML   PDF (264K)

Unit 3.21 Isolation of Intestinal Brush-Border Membranes
         Abstract | Full Text:  HTML   PDF (402K)

Unit 3.22 Isolation and Characterization of Exosomes from Cell Culture Supernatants and Biological Fluids
         Abstract | Full Text:  HTML   PDF (260K)

Unit 4.7 Cryo-Immunogold Electron Microscopy
         Abstract | Full Text:  HTML   PDF (2023K)

Unit 14.7 Analyzing FAK and Pyk2 in Early Integrin Signaling Events
         Abstract | Full Text:  HTML   PDF (330K)

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  Supplement 29, December 2005

Unit 3.15 Isolation of Lipid Droplets from Cells by Density Gradient Centrifugation
         Abstract | Full Text:  HTML   PDF (257K)

Unit 3.16 Isolation of Mast Cell Granules
         Abstract | Full Text:  HTML   PDF (472K)

Unit 3.17 Immunoisolation of Centrosomes from Drosophila melanogaster
         Abstract | Full Text:  HTML   PDF (272K)

Unit 3.18 Isolation of Zymogen Granules from Rat Pancreas
         Abstract | Full Text:  HTML   PDF (509K)

Unit 3.19 Isolation of Glyoxysomes from Pumpkin Cotyledons
         Abstract | Full Text:  HTML   PDF (313K)

Unit 11.11 Analysis of the Cell Cycle Using Xenopus Egg Extracts
         Abstract | Full Text:  HTML   PDF (163K)

Unit 21.3 Visualization of Protein Interactions in Living Cells Using Bimolecular Fluorescence Complementation ( B i FC ) Analysis
         Abstract | Full Text:  HTML   PDF (471K)

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  Supplement 28, September 2005

Unit 12.8 In Vivo Marking of Single Cells in Chick Embryos Using Photoactivation of GFP
         Abstract | Full Text:  HTML   PDF (416K)

Unit 14.3 The Detection of MAPK Signaling
         Abstract | Full Text:  HTML   PDF (445K)

Unit 23.1 Stem Cells: An Overview
         Abstract | Full Text:  HTML   PDF (488K)

Unit 23.2 Mouse Embryonic Stem Cell Derivation, and Mouse and Human Embryonic Stem Cell Culture and Differentiation as Embryoid Bodies
         Abstract | Full Text:  HTML   PDF (366K)

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  Supplement 27, June 2005

Unit 11.18 Endocytosis Assays in Intact and Permeabilized Cells
         Abstract | Full Text:  HTML   PDF (238K)

Unit 15.8 Analysis of Protein Transport to Lysosomes
         Abstract | Full Text:  HTML   PDF (156K)

Unit 18.9 Analysis of Cellular Senescence in Culture In Vivo: The Senescence-Associated -Galactosidase Assay
         Abstract | Full Text:  HTML   PDF (124K)

Unit 20.8 Inducible Gene Expression Using an Autoregulatory, Tetracycline-Controlled System
         Abstract | Full Text:  HTML   PDF (143K)

Unit 21.4 Design and Use of Fluorescent Fusion Proteins in Cell Biology
         Abstract | Full Text:  HTML   PDF (234K)

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  Supplement 26, March 2005

Unit 3.13 Isolation of Clathrin-Coated Vesicles by Differential and Density Gradient Centrifugation
         Abstract | Full Text:  HTML   PDF (1019K)

Unit 3.14 Isolation of Melanosomes
         Abstract | Full Text:  HTML   PDF (773K)

Unit 13.6 Functional Characterization of Proteins Regulating Actin Assembly
         Abstract | Full Text:  HTML   PDF (301K)

Unit 21.3 Visualization of Protein Interactions in Living Cells Using Bimolecular Fluorescence Complementation ( B i FC ) Analysis
         Abstract | Full Text:  HTML   PDF (471K)

Unit 22.9 Assembly and Micromanipulation of Xenopus In Vitro–Assembled Mitotic Chromosomes
         Abstract | Full Text:  HTML   PDF (383K)

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  Supplement 25, December 2004

Unit 3.12 Isolation of Synaptic Vesicles
         Abstract | Full Text:  HTML   PDF (372K)

Unit 4.14 Fluorescence Lifetime Imaging Microscopy
         Abstract | Full Text:  HTML   PDF (653K)

Unit 10.12 Analysis of Fibronectin Matrix Assembly
         Abstract | Full Text:  HTML   PDF (77K)

Unit 10.13 Non-Radioactive Quantification of Fibronectin Matrix Assembly
         Abstract | Full Text:  HTML   PDF (180K)

Unit 22.6 Comparative Genomic Hybridization
         Abstract | Full Text:  HTML   PDF (2824K)

Unit 22.7 Sister Chromatid Exchange
         Abstract | Full Text:  HTML   PDF (227K)

Unit 22.8 Detection of Mitotic Figures and Components of the Mitotic Machinery
         Abstract | Full Text:  HTML   PDF (107K)

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  Supplement 24, September 2004

Unit 12.7 Actin-Based Motility Assay
         Abstract | Full Text:  HTML   PDF (260K)

Unit 21.2 Fluorescence Localization After Photobleaching ( FLAP )
         Abstract | Full Text:  HTML   PDF (896K)

Unit 22.5 Multi-Color FISH Techniques
         Abstract | Full Text:  HTML   PDF (731K)

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  Supplement 23, June 2004

Unit 17.7 Chromatin Immunoprecipitation for Determining the Association of Proteins with Specific Genomic Sequences In Vivo
         Abstract | Full Text:  HTML   PDF (241K)

Unit 17.8 Isothermal Titration Calorimetry
         Abstract | Full Text:  HTML   PDF (486K)

Unit 22.1 Overview of Cytogenetic Chromosome Analysis
         Abstract | Full Text:  HTML   PDF (104K)

Unit 22.2 Preparation of Cytogenetic Specimens from Tissue Samples
         Abstract | Full Text:  HTML   PDF (186K)

Unit 22.3 Traditional Banding of Chromosomes for Cytogenetic Analysis
         Abstract | Full Text:  HTML   PDF (453K)

Unit 22.4 Fluorescence In Situ Hybridization ( FISH )
         Abstract | Full Text:  HTML   PDF (709K)

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  Supplement 22, March 2004

Unit 14.6 Determination of Akt/ PKB Signaling
         Abstract | Full Text:  HTML   PDF (272K)

Unit 15.7 Measurements of Phagocytosis and Phagosomal Maturation
         Abstract | Full Text:  HTML   PDF (384K)

Unit 17.5 Protein-Protein Interactions Identified by Pull-Down Experiments and Mass Spectrometry
         Abstract | Full Text:  HTML   PDF (679K)

Unit 17.6 Measuring Protein Interactions by Optical Biosensors
         Abstract | Full Text:  HTML   PDF (356K)

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  Supplement 21, December 2003

Unit 2.6 Preparation of Human Epidermal Keratinocyte Cultures
         Abstract | Full Text:  HTML   PDF (261K)

Unit 18.7 Nonisotopic Methods for Determination of Poly( ADP -Ribose) Levels and Detection of Poly( ADP -Ribose) Polymerase
         Abstract | Full Text:  HTML   PDF (718K)

Unit 18.8 Flow Cytometry of Apoptosis
         Abstract | Full Text:  HTML   PDF (531K)

Unit 19.6 Experimental Metastasis Assays in the Chick Embryo
         Abstract | Full Text:  HTML   PDF (568K)

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  Supplement 20, September 2003

Unit 4.11 Two-Photon Excitation Microscopy for the Study of Living Cells and Tissues
         Abstract | Full Text:  HTML   PDF (1421K)

Unit 4.12 Total Internal Reflection Fluorescence Microscopy for High-Resolution Imaging of Cell-Surface Events
         Abstract | Full Text:  HTML   PDF (431K)

Unit 4.13 Fluorescent Labeling of Yeast
         Abstract | Full Text:  HTML   PDF (525K)

Unit 18.6 Analysis of Telomeres and Telomerase
         Abstract | Full Text:  HTML   PDF (647K)

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  Supplement 19, June 2003

Unit 1.7 BY-2 Cells: Culture and Transformation for Live Cell Imaging
         Abstract | Full Text:  HTML   PDF (332K)

Unit 18.5 Analysis of Mitochondrial Dysfunction During Cell Death
         Abstract | Full Text:  HTML   PDF (360K)

Unit 20.3 Calcium Phosphate Transfection
         Abstract | Full Text:  HTML   PDF (185K)

Unit 20.4 Transfection Using DEAE -Dextran
         Abstract | Full Text:  HTML   PDF (200K)

Unit 20.5 Transfection by Electroporation
         Abstract | Full Text:  HTML   PDF (148K)

Unit 20.6 Transfection of Cultured Eukaryotic Cells Using Cationic Lipid Reagents
         Abstract | Full Text:  HTML   PDF (409K)

Unit 20.7 Optimization of Transfection
         Abstract | Full Text:  HTML   PDF (150K)

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  Supplement 18, March 2003

Unit 9.6 Measurement of Adhesion Under Flow Conditions
         Abstract | Full Text:  HTML   PDF (241K)

Unit 11.17 In Vitro RNA Splicing in Mammalian Cell Extracts
         Abstract | Full Text:  HTML   PDF (315K)

Unit 13.5 Measuring Dynamics of Nuclear Proteins by Photobleaching
         Abstract | Full Text:  HTML   PDF (249K)

Unit 19.5 The Chick Chorioallantoic Membrane as an In Vivo Angiogenesis Model
         Abstract | Full Text:  HTML   PDF (219K)

Unit 20.1 Direct Introduction of Molecules into Cells
         Abstract | Full Text:  HTML   PDF (150K)

Unit 20.2 Protein Transduction: Generation of Full-Length Transducible Proteins Using the TAT System
         Abstract | Full Text:  HTML   PDF (544K)

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  Supplement 17, January 2003

Unit 5.6 Identification of Proteins in Complex Mixtures Using Liquid Chromatography and Mass Spectrometry
         Abstract | Full Text:  HTML   PDF (308K)

Unit 10.10 Purification and Analysis of Thrombospondin-1
         Abstract | Full Text:  HTML   PDF (224K)

Unit 10.11 Purification of SPARC /Osteonectin
         Abstract | Full Text:  HTML   PDF (266K)

Unit 12.6 Optical Microscopy–Based Migration Assay for Human Neutrophils
         Abstract | Full Text:  HTML   PDF (548K)

Unit 17.4 Mapping Protein-Protein Interactions with Phage-Displayed Combinatorial Peptide Libraries
         Abstract | Full Text:  HTML   PDF (196K)

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  Supplement 16, October 2002

Unit 6.8 Fluorescence Detection of Glycoproteins in Gels and on Electroblots
         Abstract | Full Text:  HTML   PDF (218K)

Unit 6.9 Digital Electrophoresis Analysis
         Abstract | Full Text:  HTML   PDF (263K)

Unit 10.9 Preparation of Extracellular Matrices Produced by Cultured Fibroblasts

Unit 19.3 Microanalysis of Gene Expression in Tissues Using T7-SAGE: Serial Analysis of Gene Expression After High-Fidelity T7-Based RNA Amplification
         Abstract | Full Text:  HTML   PDF (350K)

Unit 19.4 SAGE Analysis from 1 µg of Total RNA
         Abstract | Full Text:  HTML   PDF (263K)

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  Supplement 15, July 2002

Unit 6.7 Agarose Gel Electrophoresis of Proteins
         Abstract | Full Text:  HTML   PDF (187K)

Unit 7.10 Radioiodination of Cellular Proteins
         Abstract | Full Text:  HTML   PDF (157K)

Unit 9.4 Analyzing Integrin-Dependent Adhesion
         Abstract | Full Text:  HTML   PDF (274K)

Appendix 3B Spectrophotometric Determination of Protein Concentration
         Abstract | Full Text:  HTML   PDF (86K)

Appendix 3H The Colorimetric Detection and Quantitation of Total Protein
         Abstract | Full Text:  HTML   PDF (238K)

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  Supplement 14, April 2002

Unit 3.11 Free-Flow Electrophoretic Analysis of Endosome Subpopulations of Rat Hepatocytes
         Abstract | Full Text:  HTML   PDF (391K)

Unit 7.9 Analysis of Oxidative Modification of Proteins
         Abstract | Full Text:  HTML   PDF (239K)

Unit 11.15 In Vitro Analysis of Peroxisomal Protein Import
         Abstract | Full Text:  HTML   PDF (178K)

Unit 11.16 In Vitro Analysis of Chloroplast Protein Import
         Abstract | Full Text:  HTML   PDF (234K)

Unit 15.6 Analysis of Protein Folding and Oxidation in the Endoplasmic Reticulum
         Abstract | Full Text:  HTML   PDF (375K)

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  Supplement 13, January 2002

Unit 4.9 Polarization Microscopy
         Abstract | Full Text:  HTML   PDF (1367K)

Unit 4.10 Fluorescent Speckle Microscopy ( FSM ) of Microtubules and Actin in Living Cells
         Abstract | Full Text:  HTML   PDF (563K)

Unit 7.8 Metabolic Labeling of Glycoproteins with Radioactive Sugars
         Abstract | Full Text:  HTML   PDF (150K)

Unit 10.8 Matrix Metalloproteinases
         Full Text:  HTML   PDF (836K)

Unit 16.6 Production of Antibodies That Recognize Specific Tyrosine-Phosphorylated Peptides
         Abstract | Full Text:  HTML   PDF (206K)

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  Supplement 12, October 2001

Unit 3.10 Isolation of Nuclei and Nuclear Membranes From Animal Tissues
         Abstract | Full Text:  HTML   PDF (174K)

Unit 7.7 Metabolic Labeling and Immunoprecipitation of Drosophila Proteins
         Abstract | Full Text:  HTML   PDF (92K)

Unit 15.5 Analysis of Membrane Traffic in Polarized Epithelial Cells
         Abstract | Full Text:  HTML   PDF (202K)

Unit 18.3 Assessment of Apoptosis and Necrosis by DNA Fragmentation and Morphological Criteria
         Abstract | Full Text:  HTML   PDF (358K)

Unit 18.4 Quantitative Fluorescence In Situ Hybridization (Q-FISH)
         Abstract | Full Text:  HTML   PDF (405K)

Unit 19.1 Overview of Metastasis Assays
         Abstract | Full Text:  HTML   PDF (218K)

Unit 19.2 Tail Vein Assay of Cancer Metastasis
         Abstract | Full Text:  HTML   PDF (186K)

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  Supplement 11, July 2001

Unit 4.8 Correlative Video Light/Electron Microscopy
         Abstract | Full Text:  HTML   PDF (408K)

Unit 9.5 Analysis of Cell-Cell Contact Mediated by Ig Superfamily Cell Adhesion Molecules
         Abstract | Full Text:  HTML   PDF (820K)

Unit 18.1 Current Concepts in Cell Death
         Abstract | Full Text:  HTML   PDF (306K)

Unit 18.2 Analysis of Caspase Activation During Apoptosis
         Abstract | Full Text:  HTML   PDF (380K)

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  Supplement 10, April 2001

Unit 2.5 Laser Capture Microdissection
         Abstract | Full Text:  HTML   PDF (299K)

Unit 3.9 Isolation of Golgi Membranes from Tissues and Cells by Differential and Density Gradient Centrifugation
         Abstract | Full Text:  HTML   PDF (221K)

Unit 11.14 Analysis of RNA Export Using Xenopus Oocytes
         Abstract | Full Text:  HTML   PDF (208K)

Unit 13.4 Movement of Nuclei
         Abstract | Full Text:  HTML   PDF (450K)

Appendix 3F Enzymatic Amplification of DNA by PCR : Standard Procedures and Optimization
         Abstract | Full Text:  HTML   PDF (153K)

Appendix 3G Micro RT-PCR
         Abstract | Full Text:  HTML   PDF (73K)

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  Supplement 9, January 2001

Unit 11.10 Preparation and Use of Interphase Xenopus Egg Extracts
         Abstract | Full Text:  HTML   PDF (333K)

Unit 11.11 Analysis of the Cell Cycle Using Xenopus Egg Extracts

Unit 11.12 Analysis of Apoptosis Using Xenopus Egg Extracts
         Abstract | Full Text:  HTML   PDF (155K)

Unit 11.13 Mitotic Spindle Assembly In Vitro
         Abstract | Full Text:  HTML   PDF (330K)

Unit 12.5 Dictyostelium Cell Dynamics
         Abstract | Full Text:  HTML   PDF (630K)

Unit 13.3 Organelle Motility in Plant Cells: Imaging Golgi and ER Dynamics with GFP
         Abstract | Full Text:  HTML   PDF (394K)

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  Supplement 8, October 2000

Unit 3.8 Isolation of Subcellular Fractions from the Yeast Saccharomyces cerevisiae
         Abstract | Full Text:  HTML   PDF (504K)

Unit 11.9 Analysis of Eukaryotic Translation in Purified and Semipurified Systems
         Abstract | Full Text:  HTML   PDF (293K)

Unit 17.3 Interaction Trap/Two-Hybrid System to Identify Interacting Proteins
         Abstract | Full Text:  HTML   PDF (464K)

Appendix 3E Silanizing Glassware
         Abstract | Full Text:  HTML   PDF (35K)

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  Supplement 7, July 2000

Unit 3.6 Isolation of Lysosomes from Tissues and Cells by Differential and Density Gradient Centrifugation
         Abstract | Full Text:  HTML   PDF (215K)

Unit 3.7 Overview of Subcellular Fractionation Procedures for the Yeast Saccharomyces cerevisiae
         Abstract | Full Text:  HTML   PDF (273K)

Unit 10.7 Proteoglycan Isolation and Analysis
         Abstract | Full Text:  HTML   PDF (166K)

Unit 17.1 Imaging Protein-Protein Interactions by Fluorescence Resonance Energy Transfer ( FRET ) Microscopy
         Abstract | Full Text:  HTML   PDF (343K)

Unit 17.2 Identification of Protein Interactions by Far Western Analysis
         Abstract | Full Text:  HTML   PDF (196K)

Appendix 1G Centrifuges and Rotors
         Abstract | Full Text:  HTML   PDF (84K)

Appendix 3D Quantification of DNA and RNA with Absorption and Fluorescence Spectroscopy
         Abstract | Full Text:  HTML   PDF (162K)

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  Supplement 6, April 2000

Unit 3.5 Isolation of Peroxisomes from Tissues and Cells by Differential and Density Gradient Centrifugation
         Abstract | Full Text:  HTML   PDF (206K)

Unit 5.5 Determination of Molecular Size by Size-Exclusion Chromatography (Gel Filtration)
         Abstract | Full Text:  HTML   PDF (234K)

Unit 6.6 Staining Proteins in Gels
         Abstract | Full Text:  HTML   PDF (210K)

Unit 7.6 Metabolic Labeling and Immunoprecipitation of Yeast Proteins
         Abstract | Full Text:  HTML   PDF (111K)

Unit 11.8 In Vitro Translation Using He L a Extract
         Abstract | Full Text:  HTML   PDF (144K)

Unit 16.5 Antibody Conjugates for Cell Biology
         Abstract | Full Text:  HTML   PDF (253K)

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  Supplement 5, January 2000

Unit 6.5 One-Dimensional Electrophoresis Using Nondenaturing Conditions
         Abstract | Full Text:  HTML   PDF (208K)

Unit 7.4 Metabolic Labeling with Fatty Acids
         Abstract | Full Text:  HTML   PDF (175K)

Unit 7.5 Metabolic Labeling of Prenyl and Carboxyl-Methyl Groups
         Abstract | Full Text:  HTML   PDF (144K)

Unit 11.7 Nuclear Import in Digitonin-Permeabilized Cells
         Abstract | Full Text:  HTML   PDF (440K)

Unit 15.4 Determining Protein Transport to the Plasma Membrane
         Abstract | Full Text:  HTML   PDF (150K)

Unit 16.3 Purification of Immunoglobulin G
         Abstract | Full Text:  HTML   PDF (171K)

Unit 16.4 Fragmentation of Immunoglobulin G
         Abstract | Full Text:  HTML   PDF (131K)

Appendix 1F Importing Biological Materials
         Abstract | Full Text:  HTML   PDF (88K)

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  Supplement 4, October 1999

Unit 1.6 Media and Culture of Yeast
         Abstract | Full Text:  HTML   PDF (177K)

Unit 3.3 Isolation of Mitochondria from Tissues and Cells by Differential Centrifugation
         Abstract | Full Text:  HTML   PDF (188K)

Unit 3.4 Purification of a Crude Mitochondrial Fraction by Density-Gradient Centrifugation
         Abstract | Full Text:  HTML   PDF (270K)

Unit 6.4 Two-Dimensional Gel Electrophoresis
         Abstract | Full Text:  HTML   PDF (291K)

Unit 7.3 Metabolic Labeling with Sulfate
         Abstract | Full Text:  HTML   PDF (99K)

Appendix 3A Molecular Biology Techniques
         Abstract | Full Text:  HTML   PDF (38K)

Appendix 3B Spectrophotometric Determination of Protein Concentration

Appendix 3C Dialysis and Concentration of Protein Solutions
         Abstract | Full Text:  HTML   PDF (166K)

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  Supplement 3, July 1999

Unit 5.4 Analysis of the Association of Proteins with Membranes
         Abstract | Full Text:  HTML   PDF (230K)

Unit 14.4 Labeling Cultured Cells with ³² P _i and Preparing Cell Lysates for Immunoprecipitation
         Abstract | Full Text:  HTML   PDF (121K)

Unit 14.5 Phosphoamino Acid Analysis
         Abstract | Full Text:  HTML   PDF (143K)

Unit 15.1 Overview of Protein Trafficking in the Secretory and Endocytic Pathways
         Abstract | Full Text:  HTML   PDF (213K)

Unit 15.2 Use of Glycosidases to Study Protein Trafficking
         Abstract | Full Text:  HTML   PDF (317K)

Unit 15.3 Endocytosis: Biochemical Analyses
         Abstract | Full Text:  HTML   PDF (190K)

Unit 16.1 Production of Monoclonal Antibodies
         Abstract | Full Text:  HTML   PDF (190K)

Unit 16.2 Production of Polyclonal Antisera
         Abstract | Full Text:  HTML   PDF (133K)

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  Supplement 2, April 1999

Unit 3.2 Isolation of Rat Hepatocyte Plasma Membrane Sheets and Plasma Membrane Domains
         Abstract | Full Text:  HTML   PDF (350K)

Unit 4.7 Cryo-Immunogold Electron Microscopy

Unit 8.5 Centrifugal Elutriation to Obtain Synchronous Populations of Cells
         Abstract | Full Text:  HTML   PDF (246K)

Unit 10.5 Purification of Fibronectin
         Abstract | Full Text:  HTML   PDF (132K)

Unit 10.6 Purification of Vitronectin
         Abstract | Full Text:  HTML   PDF (91K)

Unit 11.5 In Vitro Analysis of SV 40 DNA Replication
         Abstract | Full Text:  HTML   PDF (164K)

Unit 11.6 In Vitro Transcription
         Abstract | Full Text:  HTML   PDF (211K)

Unit 12.4 Cell Wound Assays
         Abstract | Full Text:  HTML   PDF (478K)

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  Supplement 1, January 1999

Unit 1.4 Sterilization and Filtration
         Abstract | Full Text:  HTML   PDF (231K)

Unit 1.5 Assessing and Controlling Microbial Contamination in Cell Cultures
         Abstract | Full Text:  HTML   PDF (463K)

Unit 2.4 Generation of Continuously Growing B Cell Lines by Epstein-Barr Virus Transformation
         Abstract | Full Text:  HTML   PDF (101K)

Unit 4.5 Basic Confocal Microscopy
         Abstract | Full Text:  HTML   PDF (358K)

Unit 4.6 Immunoperoxidase Methods for Localization of Antigens in Cultured Cells and Tissues
         Abstract | Full Text:  HTML   PDF (439K)

Unit 8.4 Determining Cell Cycle Stages by Flow Cytometry
         Abstract | Full Text:  HTML   PDF (279K)

Unit 9.4 Analyzing Integrin-Dependent Adhesion

Unit 10.4 Preparation of Extracellular Matrices Produced by Cultured Corneal Endothelial and PF - HR 9 Endodermal Cells
         Abstract | Full Text:  HTML   PDF (283K)

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  Core Publication, October 1998

Unit 1.1 Basic Techniques for Mammalian Cell Tissue Culture
        Full Text:  HTML   PDF (170K)

Unit 1.2 Media for Culture of Mammalian Cells
         Abstract | Full Text:  HTML   PDF (225K)

Unit 1.3 Aseptic Technique for Cell Culture
         Abstract | Full Text:  HTML   PDF (172K)

Unit 2.1 Establishment of Fibroblast Cultures
         Abstract | Full Text:  HTML   PDF (707K)

Unit 2.2 Preparation and Culture of Human Lymphocytes
         Abstract | Full Text:  HTML   PDF (166K)

Unit 2.3 Preparation of Endothelial Cells
         Abstract | Full Text:  HTML   PDF (271K)

Unit 3.1 Overview of Cell Fractionation
         Abstract | Full Text:  HTML   PDF (193K)

Unit 3.2 Isolation of Rat Hepatocyte Plasma Membrane Sheets and Plasma Membrane Domains

Unit 4.1 Proper Alignment and Adjustment of the Light Microscope
         Abstract | Full Text:  HTML   PDF (929K)

Unit 4.2 Fluorescence Microscopy
         Abstract | Full Text:  HTML   PDF (179K)

Unit 4.3 Immunofluorescence Staining
         Abstract | Full Text:  HTML   PDF (229K)

Unit 4.4 Fluorescent Staining of Subcellular Organelles: ER, Golgi Complex, and Mitochondria
         Abstract | Full Text:  HTML   PDF (503K)

Organelle Atlas: Appendix to Chapter 4
         Abstract | Full Text:  HTML   PDF (2384K)

Unit 5.1 Overview of the Physical State of Proteins Within Cells
         Abstract | Full Text:  HTML   PDF (187K)

Unit 5.2 Determining the Topology of an Integral Membrane Protein
         Abstract | Full Text:  HTML   PDF (338K)

Unit 5.3 Determination of Molecular Size by Zonal Sedimentation Analysis on Sucrose Density Gradients
         Abstract | Full Text:  HTML   PDF (311K)

Unit 6.1 One-Dimensional SDS Gel Electrophoresis of Proteins
        Full Text:  HTML   PDF (405K)

Unit 6.2 Immunoblotting and Immunodetection
         Abstract | Full Text:  HTML   PDF (245K)

Unit 6.3 Detection and Quantitation of Radiolabeled Proteins in Gels and Blots
         Abstract | Full Text:  HTML   PDF (134K)

Unit 7.1 Metabolic Labeling with Amino Acids
         Abstract | Full Text:  HTML   PDF (147K)

Unit 7.2 Immunoprecipitation
         Abstract | Full Text:  HTML   PDF (271K)

Unit 8.1 Overview of the Cell Cycle
         Abstract | Full Text:  HTML   PDF (153K)

Unit 8.2 Assays for CDK Activity and DNA Replication in the Cell Cycle
         Abstract | Full Text:  HTML   PDF (168K)

Unit 8.3 Methods for Synchronizing Cells at Specific Stages of the Cell Cycle
         Abstract | Full Text:  HTML   PDF (213K)

Unit 9.1 Cell-Substrate Adhesion Assays
         Abstract | Full Text:  HTML   PDF (140K)

Unit 9.2 Quantitative Measurement of Cell Adhesion Using Centrifugal Force
         Abstract | Full Text:  HTML   PDF (183K)

Unit 9.3 Cadherin-Dependent Cell-Cell Adhesion
         Abstract | Full Text:  HTML   PDF (162K)

Unit 10.1 Overview of Extracellular Matrix
         Abstract | Full Text:  HTML   PDF (278K)

Unit 10.2 Preparation of Basement Membrane Components from EHS Tumors
         Abstract | Full Text:  HTML   PDF (112K)

Unit 10.3 Preparation of Gelled Substrates
         Abstract | Full Text:  HTML   PDF (143K)

Unit 11.1 Overview of Eukaryotic In Vitro Translation and Expression Systems
         Abstract | Full Text:  HTML   PDF (149K)

Unit 11.2 In Vitro Translation
         Abstract | Full Text:  HTML   PDF (206K)

Unit 11.3 In Vitro Analysis of Endoplasmic-Reticulum-to-Golgi Transport in Mammalian Cells
         Abstract | Full Text:  HTML   PDF (276K)

Unit 11.4 Cotranslational Translocation of Proteins into Canine Rough Microsomes
         Abstract | Full Text:  HTML   PDF (198K)

Unit 12.1 Chemotaxis Assays for Eukaryotic Cells
         Abstract | Full Text:  HTML   PDF (871K)

Unit 12.2 Invasion Assays
         Abstract | Full Text:  HTML   PDF (159K)

Unit 12.3 Cell Traction
         Abstract | Full Text:  HTML   PDF (441K)

Unit 13.1 Microtubule/Organelle Motility Assays
         Abstract | Full Text:  HTML   PDF (263K)

Unit 13.2 In Vitro Motility Assay to Study Translocation of Actin by Myosin
         Abstract | Full Text:  HTML   PDF (118K)

Unit 14.1 Overview of Protein Phosphorylation
         Abstract | Full Text:  HTML   PDF (84K)

Unit 14.2 Immunological Detection of Phosphorylation
         Abstract | Full Text:  HTML   PDF (234K)

Unit 14.3 The Detection of MAPK Signaling

Appendix 1A Useful Measurements and Data
         Abstract | Full Text:  HTML   PDF (46K)

Appendix 1B Compendium of Drugs Commonly Used in Cell Biology Research
         Abstract | Full Text:  HTML   PDF (370K)

Appendix 1C Identification of Motifs in Protein Sequences
         Abstract | Full Text:  HTML   PDF (205K)

Appendix 1D Safe Use of Radioisotopes
         Abstract | Full Text:  HTML   PDF (179K)

Appendix 1E Absorption and Emission Maxima for Common Fluorophores
         Abstract | Full Text:  HTML   PDF (101K)

Appendix 1F Importing Biological Materials

Appendix 1G Centrifuges and Rotors

Appendix 1H Internet Basics for Biologists
         Abstract | Full Text:  HTML   PDF (164K)

Appendix 2A Common Stock Solutions, Buffers, and Media
         Abstract | Full Text:  HTML   PDF (181K)

Appendix 2B Medium Formulations
         Abstract | Full Text:  HTML   PDF (114K)

Appendix 2C Standard Laboratory Equipment
         Abstract | Full Text:  HTML   PDF (89K)

Selected Suppliers of Reagents and Equipment
         Abstract | Full Text:  HTML   PDF (180K)

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